RPS12 Human

Ribosomal Protein S12 Human Recombinant
Cat. No.
BT17162
Source
E.coli.
Synonyms
S12, 40S ribosomal protein S12.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

RPS12 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 155 amino acids (1-132 a.a.) and having a molecular mass of 16.9kDa.RPS12 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
RPS12 is a ribosomal protein which is a part of the 40S subunit. RPS12 is a member of the S12E family of ribosomal proteins. RPS12 placed in the cytoplasm. RPS12 proteins exist as multiple processed pseudogenes which are scattered throughout the genome.
Description
RPS12 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 155 amino acids (1-132 a.a.) and having a molecular mass of 16.9kDa. RPS12 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
RPS12 protein solution (0.25mg/ml) contains 20mM Tris-HCl buffer, (pH 8.0), 0.15M NaCl, and 30% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Purity
Greater than 85% as determined by SDS-PAGE.
Synonyms
S12, 40S ribosomal protein S12.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAEEGIA AGGVMDVNTA LQEVLKTALI HDGLARGIRE AAKALDKRQA HLCVLASNCD EPMYVKLVEA LCAEHQINLI KVDDNKKLGE WVGLCKIDRE GKPRKVVGCS CVVVKDYGKE SQAKDVIEEY FKCKK.

Product Science Overview

Introduction

Ribosomal Protein S12 (RPS12) is a crucial component of the ribosome, the cellular machinery responsible for protein synthesis. This protein is part of the small 40S subunit of the ribosome and belongs to the S12E family of ribosomal proteins . The human recombinant form of RPS12 is produced through recombinant DNA technology, allowing for its study and application in various research and therapeutic contexts.

Structure and Function

RPS12 is located in the cytoplasm and plays a significant role in the assembly and function of the ribosome. It is involved in the process of translating mRNA into proteins by facilitating the correct positioning of tRNA and mRNA within the ribosome . This protein is essential for maintaining the accuracy of translation, ensuring that proteins are synthesized correctly.

Biological Significance

RPS12 is not only a structural component of the ribosome but also plays a role in ribosome biogenesis. During the assembly of the small subunit processome in the nucleolus, RPS12, along with other ribosomal proteins and biogenesis factors, associates with nascent pre-rRNA. This association is crucial for RNA folding, modifications, rearrangements, and cleavage, as well as the targeted degradation of pre-ribosomal RNA by the RNA exosome .

Clinical Relevance

Increased expression of RPS12 has been observed in colorectal cancers compared to matched normal colonic mucosa . This suggests that RPS12 may have a role in cancer biology, potentially serving as a biomarker for certain types of cancer. Additionally, diseases associated with RPS12 include primary ciliary dyskinesia and fallopian tube serous papilloma .

Research Applications

The human recombinant form of RPS12 is widely used in research to study its function and role in various biological processes. By using recombinant DNA technology, researchers can produce large quantities of RPS12 for biochemical and structural studies. This allows for a deeper understanding of its function and potential therapeutic applications.

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THE BIOTEK
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