RPRD1B Human

Regulation Of Nuclear Pre-MRNA Domain Containing 1B Human Recombinant
Cat. No.
BT13030
Source
Escherichia Coli.
Synonyms
Regulation of nuclear pre-mRNA domain-containing protein 1B, C20orf77, CREPT, dJ1057B20.2, NET60.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

RPRD1B Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 349 amino acids (1-326 a.a) and having a molecular mass of 39.3kDa.
RPRD1B is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
RPRD1B, or Regulation of nuclear pre-mRNA domain-containing protein 1B, interacts with the phosphorylated C-terminal heptapeptide repeat domain (CTD) of POLR2A, the largest RNA polymerase II subunit. This interaction plays a role in CTD dephosphorylation. RPRD1B influences RNA polymerase II binding to the CCDN1 promoter, the termination region before the poly-A site, and the region after the poly-A site, promoting binding in the former two and decreasing it in the latter. It inhibits RNA polymerase II from transcribing past the 3' end termination site and potentially facilitates its return to the promoter by promoting chromatin loop formation. RPRD1B also enhances the transcription of cell cycle-related genes like CDK2, CDK4, CDK6, and cyclin-E, but not CDKN1A, CDKN1B, or cyclin-A. Furthermore, it promotes cell proliferation.
Description
Recombinant human RPRD1B, produced in E. coli, is a single, non-glycosylated polypeptide chain. It comprises 349 amino acids, with the RPRD1B sequence spanning from amino acid 1 to 326. A 23 amino acid His-tag is fused to the N-terminus. The protein has a molecular weight of 39.3 kDa and is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The RPRD1B protein is supplied as a 1 mg/ml solution in phosphate-buffered saline (pH 7.4), with 20% glycerol and 1 mM DTT.
Stability
For short-term storage (up to 4 weeks), store the vial at 4°C. For longer periods, store frozen at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of this protein is greater than 90.0%, as determined by SDS-PAGE.
Synonyms
Regulation of nuclear pre-mRNA domain-containing protein 1B, C20orf77, CREPT, dJ1057B20.2, NET60.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMSSFSES ALEKKLSELS NSQQSVQTLS LWLIHHRKHA GPIVSVWHRE LRKAKSNRKL TFLYLANDVI QNSKRKGPEF TREFESVLVD AFSHVAREAD EGCKKPLERL LNIWQERSVY GGEFIQQLKL SMEDSKSPPP KATEEKKSLK RTFQQIQEEE DDDYPGSYSP QDPSAGPLLT EELIKALQDL ENAASGDATV RQKIASLPQE VQDVSLLEKI TDKEAAERLS KTVDEACLLL AEYNGRLAAE LEDRRQLARM LVEYTQNQKD VLSEKEKKLE EYKQKLARVT QVRKELKSHI QSLPDLSLLP NVTGGLAPLP SAGDLFSTD.

Product Science Overview

Gene and Protein Structure

The RPRD1B gene is located on chromosome 20q11.23 . The gene encodes a protein consisting of 326 amino acids . The protein contains an N-terminal domain that interacts with phosphoserines in the C-terminal domain of the catalytic subunit of RNA polymerase II (POLR2A) .

Expression and Localization

RPRD1B is expressed in all adult and fetal tissues and cells examined . It is highly expressed during the early stages of mouse embryonic development and in most adult tissues . Immunohistochemical analysis has localized CREPT in the nucleus of human tumor cells .

Function and Mechanism

RPRD1B interacts with the phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A . It participates in the dephosphorylation of the CTD by RPAP2 . RPRD1B enhances the expression of cyclin D1 (CCND1) and promotes the binding of RNA polymerase II to the CCDN1 promoter and to the termination region before the poly-A site . This regulation prevents RNA polymerase II from reading through the 3’ end termination site and may allow it to be recruited back to the promoter through the promotion of chromatin loop formation .

Role in Cancer

RPRD1B has been found to be upregulated in various solid tumors, including colorectal cancer and non-small cell lung cancer . Overexpression of CREPT in several human and mouse cell lines increases cell proliferation, colony formation, and metastasis . Knockdown of CREPT has the opposite effects . CREPT specifically increases the expression of several genes controlling the cell cycle and enhances the transcription of a number of other cell cycle-related genes, including CDK2, CDK4, CDK6, and cyclin-E .

Research and Clinical Implications

The role of RPRD1B in promoting cell proliferation and its elevated expression in tumors make it a potential target for cancer therapy. Understanding the mechanisms by which RPRD1B regulates transcription and cell cycle processes can provide insights into the development of novel therapeutic strategies for cancer treatment.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.