RPRD1A Human

Regulation Of Nuclear Pre-MRNA Domain Containing 1A Human Recombinant

Cat. No.

BT12957

Source

Escherichia Coli.

Synonyms

Regulation of Nuclear Pre-MRNA Domain Containing 1A, RPRD1A, P15RS, Cyclin-Dependent Kinase 2B-Inhibitor-Related Protein, Cyclin-Dependent Kinase Inhibitor 2B-Related Protein (P15INK4B-Related Protein), P15INK4B-Related Protein, HsT3101, Regulation of Nuclear Pre-MRNA Domain-Containing Protein 1A, Cyclin-Dependent Kinase Inhibitor 2B-Related Protein, FLJ10656.

Appearance

Sterile Filtered clear solution.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

RPRD1A Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 337 amino acids (1-312) and having a molecular mass of 38.4 kDa.
RPRD1A is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

RPRD1A (Regulation of Nuclear Pre-MRNA Domain Containing 1A) shows increased expression in cells with elevated levels of cyclin-dependent kinase inhibitor p15(INK4b), suggesting a potential role in cell cycle control. This protein interacts with the phosphorylated C-terminal heptapeptide repeat domain (CTD) of RNA polymerase II subunit POLR2A, the largest subunit, and participates in the dephosphorylation of the CTD. RPRD1A may act as a negative regulator of cyclin-D1 (CCND1) and cyclin-E (CCNE1) within the cell cycle.

Description

Recombinant human RPRD1A, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 337 amino acids (residues 1-312) with a molecular weight of 38.4 kDa. The protein includes a 25 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic methods.

Physical Appearance

A clear, sterile-filtered solution.

Formulation

The RPRD1A solution is provided at a concentration of 0.5 mg/ml in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.15 M NaCl, 1 mM DTT, and 10% glycerol.

Stability

For short-term storage (up to 2-4 weeks), keep at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.

Purity

Purity is determined to be greater than 95.0% by SDS-PAGE analysis.

Synonyms

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSEFMSAFS EAALEKKLSE LSNSQQSVQT LSLWLIHHRK HSRPIVTVWE RELRKAKPNR KLTFLYLAND VIQNSKRKGP EFTKDFAPVI VEAFKHVSSE TDESCKKHLG RVLSIWEERS VYENDVLEQL KQALYGDKKP RKRTYEQIKV DENENCSSLG SPSEPPQTLD LVRALQDLEN AASGDAAVHQ RIASLPVEVQ EVSLLDKITD KESGERLSKM VEDACMLLAD YNGRLAAEID DRKQLTRMLA DFLRCQKEAL AEKEHKLEEY KRKLARVSLV RKELRSRIQS LPDLSRLPNV TGSHMHLPFA GDIYSED.

Product Science Overview

Gene and Protein Structure

The RPRD1A gene is located on chromosome 18q12.2 and spans approximately 78 kb, containing 7 exons. The protein encoded by this gene consists of 312 amino acids with a predicted molecular mass of 35.7 kDa. It contains several important domains and motifs, including:

RAR domain
Leucine zipper motif
Multiple phosphorylation sites (4 cAMP/cGMP-dependent protein kinase sites, 3 PKC sites, and 8 casein kinase II sites).

Function and Mechanism

RPRD1A is involved in the regulation of the cell cycle and transcription. It interacts with the cell cycle inhibitor cyclin-dependent kinase 4 inhibitor B (CDKN2B) and may function as a negative regulator of the G1/S phase progression. The protein forms homo- and heterodimers with Regulation Of Nuclear Pre-MRNA Domain Containing 1B (RPRD1B) to create a scaffold that interacts with the C-terminal domain of RNA polymerase II subunit B1 (POLR2A). This interaction regulates several aspects of transcription, including the dephosphorylation of the C-terminal heptapeptide repeat domain (CTD) of RNA polymerase II by RPAP2.

Expression and Regulation

RPRD1A is upregulated in cells overexpressing cyclin-dependent kinase inhibitor p15 (INK4b) and may have a role in cell cycle regulation. It is highly expressed in the prefrontal cortex, with much lower expression in other adult and fetal tissues. The protein enhances the expression of cyclin D1 (CCND1) and promotes the binding of RNA polymerase II to the CCND1 promoter.

Clinical Significance

RPRD1A has been implicated in various cellular processes and diseases. For instance, it is highly expressed in hepatocellular carcinoma (HCC) tumors and is correlated with aggressive clinicopathological features. Additionally, antisense expression of RPRD1A upregulates cyclin D1 and cyclin E in melanoma cells overexpressing transfected CDKN2B, suggesting its role as a negative regulator of these cyclins in the cell cycle.

Research and Applications

The study of RPRD1A is ongoing, with researchers exploring its potential roles in various biological processes and diseases. Understanding the function and regulation of this protein could provide insights into cell cycle control and transcription regulation, potentially leading to new therapeutic targets for cancer and other diseases.

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RPRD1A Human

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