RPLP1 Human

RPLP1 belongs to the L12P family of ribosomal proteins, which are known for their acidic nature. The protein is composed of 114 amino acids and has a molecular weight of approximately 37.9 kDa . The amino acid sequence of RPLP1 includes a series of conserved regions that are essential for its interaction with other ribosomal proteins and RNA .

Recombinant RPLP1 is typically produced using various expression systems, including E. coli and wheat germ . The protein is often tagged with GST or His-tags to facilitate purification and detection. The purification process usually involves affinity chromatography, followed by SDS-PAGE to confirm the purity and molecular weight of the protein .

RPLP1 is essential for the proper functioning of the ribosome. It is involved in the elongation phase of protein synthesis, where it helps in the accurate and efficient addition of amino acids to the growing polypeptide chain. The protein also plays a role in the regulation of ribosome assembly, ensuring that the ribosomal subunits are correctly formed and functional .

Recombinant RPLP1 is widely used in research to study ribosome function and protein synthesis. It is also used in various biochemical assays, including ELISA, Western blotting, and immunoaffinity purification . The availability of recombinant RPLP1 allows researchers to investigate its role in ribosome assembly and function, as well as its interactions with other ribosomal proteins and RNA.

Recombinant RPLP1 should be stored at -80°C to maintain its stability and prevent degradation. It is important to avoid repeated freeze-thaw cycles, which can lead to protein denaturation and loss of activity .