RPL35 Human

Ribosomal Protein L35 Human Recombinant
Cat. No.
BT16505
Source
Escherichia Coli.
Synonyms
Ribosomal Protein L35, 60S Ribosomal Protein L35, RPL35A, DBA5, L35, 60S ribosomal protein L35.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 80% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

RPL35 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 146 amino acids (1-123 a.a) and having a molecular mass of 16.9kDa.
RPL35 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Ribosomal Protein L35, also known as RPL35, is an essential component of the 60S ribosomal subunit, which is involved in protein synthesis within cells. Ribosomes, the cellular machinery responsible for protein synthesis, comprise a small 40S subunit and a larger 60S subunit. These subunits are composed of four RNA molecules and approximately 80 structurally distinct proteins. RPL35 belongs to the L29P family of ribosomal proteins and is primarily found in the cytoplasm. The gene encoding RPL35, similar to other ribosomal protein genes, has multiple processed pseudogenes dispersed throughout the genome.
Description
Recombinant RPL35 from humans, expressed in E. coli, is available as a single, non-glycosylated polypeptide chain. It consists of 146 amino acids (specifically, amino acids 1 through 123) and has a molecular weight of 16.9 kDa. This protein includes a 23-amino acid His-tag fused to its N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The RPL35 protein solution is provided at a concentration of 0.25 mg/ml. It is formulated in a buffer containing 20 mM Tris-HCl (pH 8.0) and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, freezing at -20°C is recommended. To further enhance stability during long-term storage, the addition of a carrier protein (either 0.1% HSA or BSA) is advisable. It's crucial to avoid repeated freeze-thaw cycles to maintain protein integrity.
Purity
The purity of this product is greater than 80%, as determined by SDS-PAGE analysis.
Synonyms
Ribosomal Protein L35, 60S Ribosomal Protein L35, RPL35A, DBA5, L35, 60S ribosomal protein L35.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAKIKAR DLRGKKKEEL LKQLDDLKVE LSQLRVAKVT GGAASKLSKI RVVRKSIARV LTVINQTQKE NLRKFYKGKK YKPLDLRPKK TRAMRRRLNK HEENLKTKKQ QRKERLYPLR KYAVKA.

Product Science Overview

Introduction

Ribosomal Protein L35 (RPL35) is a crucial component of the large 60S subunit of ribosomes, which are the cellular organelles responsible for protein synthesis. This protein is encoded by the RPL35 gene and belongs to the L29P family of ribosomal proteins. It is located in the cytoplasm and plays a significant role in the translation process by facilitating the assembly and function of ribosomes .

Gene and Protein Structure

The RPL35 gene is a protein-coding gene associated with several important biological pathways, including viral mRNA translation and nervous system development. The gene is located on chromosome 9 and has multiple processed pseudogenes dispersed throughout the genome . The protein itself is composed of approximately 80 structurally distinct proteins and 4 RNA species, forming a part of the large ribosomal subunit .

Function and Importance

RPL35 is essential for the proper functioning of ribosomes. It is involved in the binding of RNA and mRNA, which are critical steps in the translation process. The protein’s role in ribosome biogenesis is vital, as it contributes to the processing of pre-rRNA and the assembly of ribosomal subunits . In vivo depletion of RPL35 results in a deficit of 60S ribosomal subunits and the appearance of half-mer polysomes, indicating its importance in ribosome synthesis .

Clinical Significance

Mutations or dysregulation of the RPL35 gene have been associated with certain diseases, such as Diamond-Blackfan Anemia (DBA), a rare genetic disorder characterized by failure of the bone marrow to produce red blood cells . Understanding the function and regulation of RPL35 can provide insights into the mechanisms underlying such diseases and potentially lead to the development of targeted therapies.

Research and Applications

Recombinant RPL35 is used in various research applications to study ribosome function and protein synthesis. By expressing and purifying human recombinant RPL35, researchers can investigate its interactions with other ribosomal proteins and RNA, as well as its role in the translation process. This knowledge can contribute to the development of new therapeutic strategies for diseases related to ribosomal dysfunction.

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