RPL26 Human

Ribosomal Protein L26 Human Recombinant
Cat. No.
BT12863
Source
Escherichia Coli.
Synonyms
L26, Ribosomal Protein L26, 60S Ribosomal Protein L26.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

RPL26 Human Recombinant produced in E. coli is a single polypeptide chain containing 168 amino acids (1-145) and having a molecular mass of 19.6kDa. RPL26 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Ribosomal Protein L26, abbreviated as RPL26, is a protein found within ribosomes. It is specifically located in the larger of the two ribosomal subunits, known as the 60S subunit. This protein belongs to a family of ribosomal proteins called the L24P family and resides in the cytoplasm of the cell. Like many genes encoding ribosomal proteins, there are multiple non-functional copies of the RPL26 gene, known as pseudogenes, scattered throughout the genome.
Description
This product consists of the human RPL26 protein, manufactured in the bacterium E. coli. It is a single chain of 168 amino acids, with the first 145 amino acids forming the RPL26 protein itself. A 23 amino acid His-tag is attached to the start of the protein sequence (N-terminus). The molecular weight of the resulting protein is 19.6kDa. This recombinant RPL26 protein has been purified using specialized chromatographic methods.
Physical Appearance
A clear solution, free from any particles, that has been sterilized by filtration.
Formulation
The RPL26 is provided in a solution containing 0.25mg of protein per milliliter. The solution also contains 20mM Tris-HCl buffer with a pH of 8.0, 0.15M NaCl, 40% glycerol, and 1mM DTT.
Stability
For short-term storage (2-4 weeks), the product can be kept at a refrigerated temperature of 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure the product's stability during long-term storage, consider adding a carrier protein like HSA or BSA at a concentration of 0.1%. It's important to avoid repeatedly freezing and thawing the product.
Purity
The purity of this product is greater than 85%, as determined by SDS-PAGE analysis.
Synonyms
L26, Ribosomal Protein L26, 60S Ribosomal Protein L26.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMKFNPFV TSDRSKNRKR HFNAPSHIRR KIMSSPLSKE LRQKYNVRSM PIRKDDEVQV VRGHYKGQQI GKVVQVYRKK YVIYIERVQR EKANGTTVHV GIHPSKVVIT RLKLDKDRKK ILERKAKSRQ VGKEKGKYKE ETIEKMQE.

Product Science Overview

Introduction

Ribosomal Protein L26 (RPL26) is a crucial component of the ribosome, specifically part of the large 60S subunit. Ribosomes are essential organelles responsible for protein synthesis in cells. The human recombinant version of RPL26 is produced using recombinant DNA technology, which allows for the expression of the protein in a host organism, such as E. coli.

Structure and Function

RPL26 belongs to the L24P family of ribosomal proteins and is located in the cytoplasm . The protein consists of 145 amino acids and has a molecular weight of approximately 19.6 kDa . It plays a vital role in the assembly and function of the ribosome, contributing to the translation of mRNA into proteins .

Biological Significance

RPL26 is involved in several critical cellular processes:

  • Protein Synthesis: As a structural constituent of the ribosome, RPL26 is directly involved in the translation process, facilitating the synthesis of proteins .
  • DNA Damage Response: RPL26 has been shown to increase the translation of p53 mRNA by binding to its 5’ untranslated region (UTR) after DNA damage . This enhances the cellular response to DNA damage and helps maintain genomic stability.
  • Regulation of Apoptosis: RPL26 is implicated in the regulation of apoptosis, particularly in response to DNA damage. It positively regulates the intrinsic apoptotic signaling pathway mediated by p53 .
Recombinant Production

The human recombinant RPL26 is typically produced in E. coli and purified using conventional chromatography techniques . The recombinant protein often includes an N-terminal His-tag to facilitate purification and detection. The production process ensures high purity and functionality of the protein, making it suitable for various research applications.

Applications in Research

Recombinant RPL26 is widely used in biochemical and molecular biology research. Some of its applications include:

  • Structural Studies: Understanding the structure and function of ribosomal proteins.
  • Functional Assays: Investigating the role of RPL26 in protein synthesis and cellular responses to DNA damage.
  • Drug Development: Exploring potential therapeutic targets for diseases related to ribosomal dysfunction and DNA damage response.
Related Diseases

Mutations or dysregulation of RPL26 have been associated with certain diseases, such as Diamond-Blackfan Anemia, a rare genetic disorder characterized by failure to produce red blood cells . Understanding the function and regulation of RPL26 can provide insights into the pathogenesis of such diseases and aid in the development of targeted therapies.

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THE BIOTEK
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