ROBLD3 Human

ROBLD3 is a single, non-glycosylated polypeptide chain containing 149 amino acids, with a molecular mass of approximately 16 kDa . The recombinant human ROBLD3 protein is typically expressed in Escherichia coli (E. coli) and purified using conventional chromatography techniques . The protein often includes an N-terminal His-tag to facilitate purification and detection .

ROBLD3 functions as an adapter protein that enhances the efficiency of the MAP kinase cascade. It facilitates the activation of MAPK2 by recruiting MP1 to late endosomes, where they form a stable heterodimeric complex required for ERK activation on endosomes . This interaction is essential for the proper functioning of the MAPK pathway, which is involved in various cellular processes, including growth, differentiation, and response to external stimuli.

In humans, mutations in the ROBLD3 gene have been associated with primary immunodeficiency syndrome, indicating its role in endosomal biogenesis and immune system function . The protein’s involvement in the MAPK pathway also suggests its importance in cellular signaling and regulation.

Recombinant human ROBLD3 protein is used in various research applications, including studies on cellular signaling pathways, protein-protein interactions, and the development of therapeutic interventions for diseases related to MAPK pathway dysregulation . The protein’s high purity and stability make it a valuable tool for biochemical and structural studies.

The recombinant human ROBLD3 protein is typically stored at 4°C for short-term use and at -20°C for long-term storage. It is important to avoid freeze-thaw cycles to maintain the protein’s integrity and functionality .