RNMTL1 Human

RNA Methyltransferase Like 1 Human Recombinant
Cat. No.
BT13328
Source
Escherichia Coli.
Synonyms
RNA Methyltransferase Like 1, 16S RRNA (Guanosine(1370)-2-O)-Methyltransferase, 16S RRNA [Gm1370] 2-O-Methyltransferase, RNA Methyltransferase-Like Protein 1, HC90, MRM3, RRNA Methyltransferase 3, Mitochondrial, Putative RNA Methyltransferase, EC 2.1.1.-, EC 2.1.1, RMTL1, RNMTL1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

RNMTL1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 443 amino acids (1-420 a.a) and having a molecular mass of 49.4kDa.
RNMTL1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
RNA methyltransferase-like protein 1, also known as RNMTL1, is a member of the RNA methyltransferase TrmH family. It is a probable RNA methyltransferase and is expressed at equal levels in both normal liver cells and hepatocellular carcinoma cells.
Description
Recombinant human RNMTL1 protein, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 443 amino acids (with amino acids 1 to 420 included). It has a molecular weight of 49.4 kDa. A 23-amino acid His-tag is fused to the N-terminus of the RNMTL1 protein. Purification is achieved using proprietary chromatographic methods.
Physical Appearance
A sterile, colorless solution that has been filtered.
Formulation
This solution contains 1 mg/mL of RNMTL1 protein, 20 mM Tris-HCl with a pH of 8.0, and 10% glycerol.
Stability
For optimal storage, keep the entire vial at 4°C if it will be used within 2 to 4 weeks. For long-term storage, freeze the solution at -20°C. Adding a carrier protein, such as 0.1% HSA or BSA, is recommended for extended storage. It is important to avoid repeated cycles of freezing and thawing.
Purity
SDS-PAGE analysis has determined the purity to be greater than 85.0%.
Synonyms
RNA Methyltransferase Like 1, 16S RRNA (Guanosine(1370)-2-O)-Methyltransferase, 16S RRNA [Gm1370] 2-O-Methyltransferase, RNA Methyltransferase-Like Protein 1, HC90, MRM3, RRNA Methyltransferase 3, Mitochondrial, Putative RNA Methyltransferase, EC 2.1.1.-, EC 2.1.1, RMTL1, RNMTL1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAALVRP ARFVVRPLLQ VVQAWDLDAR RWVRALRRSP VKVVFPSGEV VEQKRAPGKQ PRKAPSEASA QEQREKQPLE ESASRAPSTW EESGLRYDKA YPGDRRLSSV MTIVKSRPFR EKQGKILLEG RRLISDALKA GAVPKMFFFS RLEYLKELPV DKLKGVSLIK VKFEDIKDWS DLVTPQGIMG IFAKPDHVKM TYPKTQLQHS LPLLLICDNL RDPGNLGTIL RSAAGAGCSK VLLTKGCVDA WEPKVLRAGM GAHFRMPIIN NLEWETVPNY LPPDTRVYVA DNCGLYAQAE MSNKASDHGW VCDQRVMKFH KYEEEEDVET GASQDWLPHV EVQSYDSDWT EAPAAVVIGG ETYGVSLESL QLAESTGGKR LLIPVVPGVD SLNSAMAASI LLFEGKRQLR GRAEDLSRDR SYH

Product Science Overview

Introduction

RNA Methyltransferase Like 1 (METTL1) is an enzyme that plays a crucial role in the post-transcriptional modification of RNA. It is responsible for the methylation of RNA molecules, specifically the N7-methylguanosine (m7G) modification on tRNA. This modification is essential for the proper functioning of tRNA, influencing its stability and the efficiency of protein synthesis.

Function and Importance

METTL1, along with its cofactor WDR4, forms a complex that catalyzes the m7G modification on tRNA. This modification is critical for the proper folding and structural stability of tRNA, which in turn affects the overall efficiency of protein synthesis. The m7G modification is also involved in the regulation of gene expression and has been linked to various cellular processes, including cell proliferation and differentiation.

Role in Disease

Recent studies have highlighted the role of METTL1 in various diseases, particularly in cancer. Aberrant expression of METTL1 has been associated with the development and progression of several types of cancer, including acute myeloid leukemia (AML). In AML, elevated levels of METTL1 and WDR4 have been linked to poor prognosis. Knockdown of METTL1 in AML cells has been shown to reduce cell proliferation and increase apoptosis, suggesting that METTL1 could be a potential therapeutic target for AML .

Research and Applications

Research on METTL1 has provided valuable insights into the mechanisms of RNA modification and its impact on cellular processes. In vitro assays for RNA methyltransferase activity have been developed to study the target specificity, catalytic activity, and function of METTL1 and other RNA methyltransferases . These assays are essential for understanding the role of RNA modifications in health and disease and for developing potential therapeutic strategies.

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