RNH1 Antibody
Description
Product Specs
Product Science Overview
Ribonuclease inhibitors (RIs) are proteins that play a crucial role in regulating the activity of ribonucleases (RNases), enzymes that degrade RNA. The mouse anti-human ribonuclease inhibitor is a monoclonal antibody designed to inhibit human ribonucleases, particularly angiogenin, which is involved in various physiological processes, including angiogenesis and tumor growth .
Ribonuclease inhibitors are characterized by their leucine-rich repeat (LRR) structure, which forms a horseshoe shape. This structure allows RIs to bind tightly to ribonucleases, preventing them from degrading RNA. The binding affinity between RIs and RNases is among the highest for any protein-protein interaction, with dissociation constants in the femtomolar range .
Ribonuclease inhibitors were first discovered in the 1960s in rat liver homogenates. They were found to bind and inhibit pancreatic-type RNases such as RNase A, RNase 1, RNase 2, RNase 4, and angiogenin (RNase 5). The evolutionary conservation of RIs among mammals suggests their significant role in cellular processes .
RIs are involved in various biological functions beyond RNA protection. They play roles in cancer growth and metastasis, microRNA processing, differentiation and myelination of oligodendrocytes, and inhibition of oxidative damage. Recent studies have shown that RIs are essential for embryonic development, mRNA translation, hematopoiesis, and inflammation .
The mouse anti-human ribonuclease inhibitor is a monoclonal antibody derived from hybridizing mouse myeloma cells with spleen cells from immunized mice. This antibody specifically targets human ribonucleases, inhibiting their activity and thereby protecting RNA from degradation. It has been used in various research applications, including studies on cancer and other diseases .
RIs are widely used in biochemical reactions to safeguard RNA from degradation by unintended RNases. The mouse anti-human ribonuclease inhibitor has been employed in research to study the role of ribonucleases in cancer and other diseases. It has shown potential in suppressing tumor growth in animal models, highlighting its therapeutic potential .
