RNF34 Human

Ring Finger Protein 34 Human Recombinant
Cat. No.
BT18450
Source
Escherichia Coli.
Synonyms
Ring Finger Protein 34, E3 Ubiquitin Protein Ligase, RING Finger Protein 34, Caspase Regulator CARP1, Caspases-8 And -10-Associated RING Finger Protein 1, FYVE-RING Finger Protein Momo, Human RING Finger Homologous To Inhibitor Of Apoptosis Protein, CARP-1, hRFI, RING Finger Protein RIFF, RFI, CARP1, RIF, RIFF, E3 Ubiquitin-Protein Ligase RNF34, EC 6.3.2.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

RNF34 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 396 amino acids (1-373a.a) and having a molecular mass of 44.2kDa.
RNF34 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Ring Finger Protein 34 (RNF34) is an E3 ubiquitin-protein ligase, meaning it plays a role in protein degradation. It features a RINF finger motif, known for its involvement in protein-protein and protein-DNA interactions. RNF34 regulates the levels of CASP8 and CASP10 proteins by targeting them for breakdown by the proteasome. Moreover, RNF34 protects cells from apoptosis (programmed cell death) induced by TNF. It also interacts with phosphatidylinositol 5-phosphate and phosphatidylinositol 3-phosphate. Alternative splicing of the RNF34 gene results in multiple transcript variants that encode different isoforms of the protein.
Description
Recombinant human RNF34, produced in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 396 amino acids (1-373a.a) and has a molecular weight of 44.2 kDa. The protein is fused to a 23 amino acid His-tag at its N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, sterile-filtered solution.
Formulation
The RNF34 protein solution is provided at a concentration of 1 mg/ml. It is formulated in a buffer containing 20 mM Tris-HCl (pH 8.0), 10% glycerol, 0.1 M NaCl, and 1 mM DTT.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is advisable for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of RNF34 is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
Ring Finger Protein 34, E3 Ubiquitin Protein Ligase, RING Finger Protein 34, Caspase Regulator CARP1, Caspases-8 And -10-Associated RING Finger Protein 1, FYVE-RING Finger Protein Momo, Human RING Finger Homologous To Inhibitor Of Apoptosis Protein, CARP-1, hRFI, RING Finger Protein RIFF, RFI, CARP1, RIF, RIFF, E3 Ubiquitin-Protein Ligase RNF34, EC 6.3.2.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGS MRKAGAT SMWASCCGLL NEVMGTGAVR GQQSAFAGAT GPFRFTPNPE FSTYPPAATE GPNIVCKACG LSFSVFRKKH VCCDCKKDFC SVCSVLQENL RRCSTCHLLQ ETAFQRPQLM RLKVKDLRQY LILRNIPIDT CREKEDLVDL VLCHHGLGSE DDMDTSSLNS SRSQTSSFFT RSFFSNYTAP SATMSSFQGE LMDGDQTSRS GVPAQVQSEI TSANTEDDDD DDDEDDDDEE ENAEDRNPGL SKERVRASLS DLSSLDDVEG MSVRQLKEIL ARNFVNYSGC CEKWELVEKV NRLYKENEEN QKSYGERLQL QDEEDDSLCR ICMDAVIDCV LLECGHMVTC TKCGKRMSEC PICRQYVVRA VHVFKS

Product Science Overview

Structure and Expression

RNF34 is a single, non-glycosylated polypeptide chain containing 396 amino acids and has a molecular mass of approximately 44.2 kDa . The protein is expressed in various tissues and is involved in several cellular processes, including the regulation of apoptosis and protein degradation.

Biological Functions

RNF34 functions primarily as an E3 ubiquitin-protein ligase, which means it plays a crucial role in the ubiquitination process. Ubiquitination is a post-translational modification where ubiquitin proteins are attached to a substrate protein, marking it for degradation by the proteasome. This process is essential for maintaining cellular homeostasis by regulating the levels of various proteins.

One of the key roles of RNF34 is to regulate the levels of caspase-8 (CASP8) and caspase-10 (CASP10) by targeting them for proteasomal degradation . Caspases are a family of protease enzymes that play essential roles in programmed cell death (apoptosis). By controlling the degradation of these caspases, RNF34 helps protect cells against apoptosis induced by tumor necrosis factor (TNF).

Interaction with Phosphoinositides

RNF34 also binds to phosphatidylinositol 5-phosphate and phosphatidylinositol 3-phosphate . These interactions suggest that RNF34 may have additional roles in cellular signaling pathways, particularly those involving phosphoinositides, which are important lipid signaling molecules.

Recombinant Production

Recombinant human RNF34 is produced using various expression systems, including Escherichia coli and baculovirus-insect cells . The recombinant protein is typically purified using chromatographic techniques to ensure high purity and biological activity. It is supplied in a sterile solution containing Tris-HCl buffer, glycerol, NaCl, and DTT to maintain stability and activity .

Applications

Recombinant RNF34 is used in various research applications, including studies on protein-protein interactions, apoptosis, and ubiquitination. It is also valuable for investigating the regulatory mechanisms of caspases and the role of phosphoinositides in cellular signaling.

Storage and Stability

For optimal storage, recombinant RNF34 should be kept at -70°C to maintain its stability and biological activity. It is recommended to aliquot the protein into smaller quantities to avoid repeated freeze-thaw cycles, which can degrade the protein .

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