RNF114 Human
Description
RNF114 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
RNF114 contains both RING and C2HC type zinc finger domains . The RING (Really Interesting New Gene) finger domain is a specialized type of zinc finger that binds two zinc ions and is involved in mediating protein-protein interactions. The C2HC type zinc finger domain is another type of zinc finger that also binds zinc ions and plays a role in DNA binding and protein-protein interactions.
RNF114 is involved in several critical cellular processes:
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Cell Cycle Regulation: RNF114 activates cell cycle progression and suppresses cellular senescence by ubiquitinating and destabilizing cyclin-dependent kinase inhibitors such as p21 WAF1, p27 KIP1, and p57 KIP2 . This activity is crucial for the G1-to-S phase transition in the cell cycle.
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Immune Response: RNF114 acts as a negative regulator of NF-kappa-B-dependent transcription by promoting the ubiquitination and stabilization of the NF-kappa-B inhibitor TNFAIP3 . It also inhibits cellular double-stranded RNA responses and interferon production by targeting the MAVS component for proteasomal degradation .
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Cancer Research: Nimbolide, a terpenoid small molecule derived from Neem trees, interferes with RNF114-dependent ubiquitination of p21, resulting in its rapid stabilization. This has been shown to impair the proliferation of breast cancer cells and is being used as a basis for the development of targeted protein degradation therapies .
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Spermatogenesis: RNF114 is expressed in various tissues, including skin, CD4 lymphocytes, and dendritic cells, and plays a role in spermatogenesis .
Recombinant human RNF114 is produced using E. coli expression systems. The recombinant protein is typically purified to a high degree of purity, often greater than 85%, as determined by SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining . The predicted molecular mass of the recombinant protein is approximately 26 kDa, but it often appears as a 27 kDa band on SDS-PAGE due to post-translational modifications .
Recombinant human RNF114 is used in various research applications, including:
- Cell and Tissue Culture: As a standard in ELISA assays or for studying the effects of RNF114 on cell cycle progression and immune response.
- Cancer Research: Investigating the role of RNF114 in cancer cell proliferation and the development of targeted therapies.
- Protein Degradation Studies: Understanding the mechanisms of ubiquitination and proteasomal degradation.
Recombinant human RNF114 should be stored at -70°C to maintain its stability. It is recommended to use a manual defrost freezer and avoid repeated freeze-thaw cycles. Under sterile conditions, the protein can be stored for up to 48 months from the date of receipt at -70°C and for up to 3 months after opening .
