RLBP1 Human, sf9

Retinaldehyde Binding Protein 1 Human Recombinant, sf9
Cat. No.
BT12529
Source
Sf9, Baculovirus cells.
Synonyms
Retinaldehyde-binding protein 1, Cellular retinaldehyde-binding protein, RLBP1, CRALBP.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

RLBP1 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 326 amino acids (1-317) and having a molecular mass of 37.5kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa).
RLBP1 is fused to 6 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
RLBP1, a 36-kD water-soluble protein, functions as a transporter for 11-cis-retinal or 11-cis-retinaldehyde, playing a crucial role in the visual cycle. Mutations in the RLBP1 gene are linked to retinal disorders such as retinitis punctata albescens, severe rod-cone dystrophy, and Bothnia dystrophy, a non-syndromic autosomal recessive form of retinitis pigmentosa.
Description
Recombinant human RLBP1, expressed in Sf9 insect cells using a baculovirus system, is a single, glycosylated polypeptide chain. It consists of 326 amino acids (residues 1-317) with a molecular mass of 37.5 kDa. On SDS-PAGE analysis, the apparent molecular size will be approximately 28-40 kDa due to glycosylation. The protein has a C-terminal 6-His tag for purification purposes and is purified using proprietary chromatographic methods.
Physical Appearance
Clear, sterile-filtered solution.
Formulation
The RLBP1 protein is supplied as a 0.5 mg/ml solution in phosphate-buffered saline (pH 7.4) containing 20% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the protein can be stored at 4°C. For extended storage, it is recommended to freeze the protein at -20°C. To ensure long-term stability during frozen storage, the addition of a carrier protein such as 0.1% HSA or BSA is advisable. Avoid repeated freeze-thaw cycles to maintain protein integrity.
Purity
The purity of RLBP1 is greater than 90% as determined by SDS-PAGE analysis.
Synonyms
Retinaldehyde-binding protein 1, Cellular retinaldehyde-binding protein, RLBP1, CRALBP.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
ADPMSEGVGT FRMVPEEEQE LRAQLEQLTT KDHGPVFGPC SQLPRHTLQK AKDELNEREE TREEAVRELQ EMVQAQAASG EELAVAVAER VQEKDSGFFL RFIRARKFNV GRAYELLRGY VNFRLQYPEL FDSLSPEAVR CTIEAGYPGV LSSRDKYGRV VMLFNIENWQ SQEITFDEIL QAYCFILEKL LENEETQING FCIIENFKGF TMQQAASLRT SDLRKMVDML QDSFPARFKA IHFIHQPWYF TTTYNVVKPF LKSKLLERVF VHGDDLSGFY QEIDENILPS DFGGTLPKYD GKAVAEQLFG PQAQAENTAF HHHHHH.

Product Science Overview

Introduction

Retinaldehyde Binding Protein 1 (RLBP1), also known as Cellular Retinaldehyde-Binding Protein (CRALBP), is a crucial protein in the visual cycle. This protein is encoded by the RLBP1 gene in humans and plays a significant role in the metabolism of retinoids, which are vital for vision.

Structure and Characteristics

RLBP1 is a 36-kilodalton (kDa) water-soluble protein that carries 11-cis-retinaldehyde or 11-cis-retinal as its physiological ligands . The human recombinant version of this protein, produced in Sf9 Baculovirus cells, consists of a single, glycosylated polypeptide chain containing 326 amino acids (1-317) and has a molecular mass of approximately 37.5 kDa .

Discovery and Function

The cellular retinaldehyde-binding protein was first discovered in 1977 after being purified from retina and retinal pigment epithelial cells . RLBP1 participates in the regeneration of active 11-cis-retinol and 11-cis-retinaldehyde from the inactive 11-trans products of the rhodopsin photocycle. This process is part of the visual cycle, which is essential for visual perception .

Tissue Distribution

RLBP1 is predominantly found in the retina and retinal pigment epithelial cells. However, it is also expressed in other tissues such as the iris, cornea, ciliary epithelium, Muller cells, pineal gland, and oligodendrocytes of the optic nerve and brain . The function of RLBP1 in non-ocular tissues is not yet fully understood.

Clinical Significance

Mutations in the RLBP1 gene can lead to various retinal dystrophies, including retinitis pigmentosa and Bothnia dystrophy. These conditions are characterized by progressive vision loss due to the degeneration of photoreceptor cells in the retina .

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