Rhodanese Human
Description
Product Specs
Product Science Overview
The gene encoding thiosulfate sulfurtransferase in humans is known as TST and is located on chromosome 22q12.3 . The TST gene produces a protein that contains two rhodanese domains, which are essential for its enzymatic activity . The protein is localized to the mitochondria, where it catalyzes the conversion of thiosulfate and cyanide to thiocyanate and sulfite .
Thiosulfate sulfurtransferase is involved in several critical biochemical processes:
- Cyanide Detoxification: The enzyme converts toxic cyanide into less harmful thiocyanate, which can be excreted from the body .
- Sulfur and Selenium Transport: It facilitates the transport of sulfur and selenium in biologically available forms .
- Iron-Sulfur Cluster Formation: The enzyme plays a role in the formation and maintenance of iron-sulfur clusters, which are essential for various cellular functions .
- Redox System Maintenance: It helps maintain the redox balance within cells .
- Mitochondrial Import of 5S rRNA: Thiosulfate sulfurtransferase interacts with 5S ribosomal RNA and aids its import into the mitochondria .
The enzyme’s ability to detoxify cyanide and its involvement in sulfur metabolism make it a valuable target for biomedical research. Thiosulfate sulfurtransferase has potential applications in treating metabolic diseases such as diabetes . Additionally, its role in maintaining redox balance and forming iron-sulfur clusters highlights its importance in cellular health and function .
Human recombinant thiosulfate sulfurtransferase is produced using genetic engineering techniques. The TST gene is cloned into an expression vector, which is then introduced into a host organism, such as bacteria or yeast. The host organism produces the enzyme, which can be purified and used for research or therapeutic purposes.
