RFXANK Human

Regulatory Factor X-Associated Ankyrin-Containing Protein Human Recombinant

Cat. No.

BT12108

Source

Escherichia Coli.

Synonyms

ANKRA1, BLS, F14150_1, RFX-B, DNA-binding protein RFXANK, Ankyrin repeat family A protein 1, Regulatory factor X subunit B, Regulatory factor X-associated ankyrin-containing protein, RFXANK, RFXB.

Appearance

Sterile Filtered clear solution.

Purity

Greater than 85% as determined by SDS-PAGE.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

RFXANK Human Recombinant produced in E. coli is a single polypeptide chain containing 260 amino acids (1-237) and having a molecular mass of 28kDa. RFXANK is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

Regulatory Factor X-Associated Ankyrin-Containing Protein (RFXANK) is a protein that plays a crucial role in the immune system by regulating the expression of MHC class II genes. These genes are essential for the immune system's ability to recognize and respond to foreign invaders. RFXANK contains ankyrin repeats, which are protein-protein interaction domains that allow it to interact with other proteins involved in MHC class II gene regulation. Notably, RFXANK forms a complex with regulatory factor X-associated protein and regulatory factor-5. This complex binds to a specific DNA sequence called the X box motif, located within the promoter regions of MHC class II genes. This binding is critical for initiating the transcription of these genes. Once bound to the promoter, the RFXANK complex recruits another protein called MHC class II transactivator, which further enhances the expression of MHC class II genes. This intricate regulatory mechanism ensures that MHC class II genes are expressed only in specific cell types and under appropriate conditions. Mutations in the RFXANK gene can disrupt this delicate balance, leading to a rare genetic disorder known as bare lymphocyte syndrome type II, complementation group B. This condition severely compromises the immune system, making individuals highly susceptible to infections.

Description

Recombinant human RFXANK, expressed in E. coli, is a single polypeptide chain with a molecular weight of 28 kDa. It encompasses amino acids 1-237 of the native protein and includes a 23 amino acid His-tag at the N-terminus to facilitate purification. The protein is purified using proprietary chromatographic techniques.

Physical Appearance

Clear, colorless solution, sterile-filtered.

Formulation

RFXANK is supplied as a 0.25 mg/ml solution in a buffer consisting of 20mM Tris-HCl (pH 8.0), 0.15M NaCl, and 20% glycerol.

Stability

For short-term storage (up to 4 weeks), keep at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.

Purity

Purity is determined to be greater than 85% by SDS-PAGE analysis.

Synonyms

ANKRA1, BLS, F14150_1, RFX-B, DNA-binding protein RFXANK, Ankyrin repeat family A protein 1, Regulatory factor X subunit B, Regulatory factor X-associated ankyrin-containing protein, RFXANK, RFXB.

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMELTQPA EDLIQTQQTP ASELGDPEDP GEEAADGSDT VVLSLFPCTP EPVNPEPDAS VSSPQGSSLK HSTTLTNRQR GNEVSALPAT LDCDNLVNKP DERGFTPLIW ASAFGEIETV RFLLEWGADP HILAKERESA LSLASTGGYT DIVGLLLERD VDINIYDWNG GTPLLYAVRG NHVKCVEALL ARGADLTTEA DSGYTPMDLA VALGYRKVQQ VIENHILKLF QSNLVPADPE.

Product Science Overview

Introduction

Regulatory Factor X-Associated Ankyrin-Containing Protein (RFXANK) is a crucial component in the regulation of major histocompatibility complex (MHC) class II gene expression. This protein is encoded by the RFXANK gene and plays a significant role in the immune system by influencing the transcription of MHC class II molecules, which are essential for the immune response.

Gene and Protein Structure

The RFXANK gene is located on chromosome 19 in humans. The protein encoded by this gene contains three ankyrin repeats, which are known for their role in protein-protein interactions. These ankyrin repeats are essential for the formation of the regulatory factor X (RFX) complex, which is necessary for binding to the X box motif of MHC class II gene promoters.

Function

RFXANK, along with regulatory factor X-associated protein (RFXAP) and regulatory factor-5 (RFX5), forms the RFX complex. This complex binds to the X box motif of certain MHC class II gene promoters and activates their transcription . The activation of these promoters is crucial for the expression of MHC class II molecules, which present antigens to T cells and initiate an immune response.

Once the RFX complex binds to the promoter, it associates with the non-DNA-binding factor MHC class II transactivator (CIITA), which controls the cell type specificity and inducibility of MHC class II gene expression. This interaction ensures that MHC class II molecules are expressed in the appropriate cells and under the right conditions.

Clinical Significance

Mutations in the RFXANK gene have been linked to bare lymphocyte syndrome type II, complementation group B . This rare genetic disorder is characterized by a lack of MHC class II expression, leading to severe immunodeficiency. Patients with this condition are highly susceptible to infections due to the impaired ability of their immune system to present antigens and activate T cells.

Recombinant RFXANK

Human recombinant RFXANK is produced using recombinant DNA technology, which involves inserting the RFXANK gene into a suitable expression system, such as bacteria or yeast, to produce the protein in large quantities. This recombinant protein can be used for various research purposes, including studying the function of RFXANK in the immune system and developing potential therapies for conditions related to MHC class II deficiency.

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RFXANK Human

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