REXO1 Human
RNA Exonuclease 1 Human Recombinant
Cat. No.
BT14211
Source
Escherichia Coli.
Synonyms
RNA exonuclease 1 homolog, Elongin-A-binding protein 1, EloA-BP1, Transcription elongation factor B polypeptide 3-binding protein 1, REXO1, ELOABP1, KIAA1138, TCEB3BP1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
REXO1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 198 amino acids (1060-1221a.a) and having a molecular mass of 22.3kDa. REXO1 is fused to a 36 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
REXO1, also known as RNA exonuclease 1 homolog, is a protein involved in RNA metabolism. It interacts with the Elongin complex, which plays a crucial role in regulating transcription elongation by RNA polymerase II. This complex enhances transcription by reducing pausing of the polymerase during its movement along the DNA template. REXO1, consisting of 1221 amino acids, is widely expressed in various tissues. Its presence is particularly notable in embryonic and adult stem cells, suggesting its potential importance in stem cell biology.
Description
This product consists of a recombinant human REXO1 protein produced in E. coli. It is a single polypeptide chain that lacks glycosylation and comprises 198 amino acids (spanning positions 1060 to 1221). The protein has a molecular weight of 22.3 kDa. For purification and detection purposes, a 36-amino acid His-tag is fused to the N-terminus of the protein. Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
The product is a sterile, colorless solution that has been filtered for clarity.
Formulation
The REXO1 protein is supplied in a solution at a concentration of 0.25 mg/ml. The solution is buffered with 20 mM Tris-HCl at pH 8.0 and contains 0.15 M NaCl, 10% glycerol, and 1 mM DTT.
Stability
For short-term storage (up to 4 weeks), the product can be kept at 4°C. For extended storage, it is recommended to freeze the product at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is advisable for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity of the REXO1 protein is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
RNA exonuclease 1 homolog, Elongin-A-binding protein 1, EloA-BP1, Transcription elongation factor B polypeptide 3-binding protein 1, REXO1, ELOABP1, KIAA1138, TCEB3BP1.
Source
Escherichia Coli.
Amino Acid Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSIYAL DCEMSYTTYG LELTRVTVVD TDVHVVYDTF VKPDNEIVDY NTRFSGVTEA DLADTSVTLR DVQAVLLSMF SADTILIGHS LESDLLALKV IHSTVVDTSV LFPHRLGLPY KRSLRNLMAD YLRQIIQDNV DGHSSSEDAG ACMHLVIWKV REDAKTKR.
Product Science Overview
Structure and Function
EXO1 is a member of the Rad2 nuclease family and is composed of 803 amino acids . It shares 55% similarity with its yeast counterpart, Exo1 . The enzyme plays a crucial role in several cellular processes, including:
- DNA Recombination: EXO1 is involved in the recombination of direct repeat DNA fragments, which is essential for genetic diversity and repair .
- RNA Primer Removal: During DNA replication, EXO1 helps remove RNA primers from Okazaki fragments on the lagging strand .
- Mutation Avoidance: By participating in DNA mismatch repair, EXO1 helps maintain genomic stability and prevent mutations that could lead to diseases like cancer .
Biological Importance
EXO1’s role in DNA repair is vital for maintaining the integrity of the genome. Dysfunctional mutations in DNA mismatch repair genes, such as EXO1, can lead to hereditary non-polyposis colorectal cancer and other sporadic cancers . Additionally, EXO1’s involvement in RNA primer removal during DNA replication highlights its importance in ensuring accurate DNA synthesis .
Industrial and Research Applications
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