RCVRN Mouse
RCV1, Cancer-associated retinopathy protein, Protein CAR, RCVRN, Recoverin, S-modulin.
Greater than 90.0% as determined by SDS-PAGE.
Description
Recoverin Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 225 amino acids (1-202a.a.) and having a molecular mass of 25.8kDa. Recoverin Mouse is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
RCV1, Cancer-associated retinopathy protein, Protein CAR, RCVRN, Recoverin, S-modulin.
MGSSHHHHHH SSGLVPRGSH MGSMGNSKSG ALSKEILEEL QLNTKFTEEE LSAWYQSFLK ECPSGRITRQ EFESIYSKFF PDSDPKAYAQ HVFRSFDANS DGTLDFKEYV IALHMTTAGK PTQKLEWAFS LYDVDGNGTI SKNEVLEIVM AIFKMIKPED VKLLPDDENT PEKRAEKIWA FFGKKEDDKL TEEEFIEGTL ANKEILRLIQ FEPQKVKERI KEKKQ.
Product Science Overview
Recoverin is a low molecular-weight, neuronal calcium sensor (NCS) protein primarily located in the photoreceptor outer segments of the vertebrate retina . It plays a crucial role in the recovery phase of visual excitation and adaptation to background light . This article delves into the historical background, structure, function, and significance of Recoverin, particularly focusing on the mouse recombinant version.
The discovery of Recoverin dates back to 1989 when P. Philippov’s group from M.V. Lomonosov Moscow State University developed a method for purifying visual G-protein transducin and other G-proteins . During this process, they identified an unknown protein with an apparent molecular weight of 26 kDa, which they named "p26" . This protein was later found to be specific to the retina, particularly the photoreceptor layer, and was capable of binding calcium ions (Ca²⁺) due to its EF-hand type calcium-binding sites . The protein was subsequently renamed “Recoverin” due to its role in photoreceptor recovery .
Recoverin contains several EF-hand type Ca²⁺-binding sites, which are crucial for its function . The binding of calcium to these sites induces a conformational change in the protein, enabling it to interact with other molecules . Recoverin also undergoes N-terminal myristoylation, a lipid modification that facilitates its attachment to cellular membranes .
Recoverin plays a pivotal role in the visual cycle by acting as a calcium sensor . In the dark, calcium-bound Recoverin inhibits G-protein-coupled receptor kinase (GRK), preventing the phosphorylation of rhodopsin, a visual receptor . This inhibition is lifted when light reduces intracellular calcium levels, allowing GRK to phosphorylate rhodopsin and initiate the recovery phase of visual excitation . Recoverin’s ability to bind to phospholipid membranes further enhances its regulatory functions .
Recoverin is not only essential for normal visual function but also serves as a marker for evaluating the differentiation of pluripotent stem cells into rod and cone photoreceptors or cone bipolar cells . Additionally, Recoverin is classified as a cancer-retina antigen due to its aberrant expression in tumors of the lung and other tissues . This makes it a valuable tool in both basic research and clinical diagnostics.
