RAP Rat

Recombinant Rat Receptor Associated Protein is produced in Escherichia coli (E. coli) and is a single, non-glycosylated polypeptide chain. It consists of 327 amino acids and has a molecular mass of approximately 38,862 Daltons . The recombinant version often includes tags such as 6xHis and 1xC-myc for purification purposes .

RAP functions by binding to LDLR family members, preventing their premature interaction with ligands within the endoplasmic reticulum (ER). This ensures that the receptors are correctly folded and transported to their proper cellular locations. One of the key roles of RAP is to avert the glial cell-mediated (GCM) enhancement of axon growth by ApoE-containing lipoproteins . In the presence of RAP, the growth stimulatory effect of these lipoproteins is annulled, indicating that the effect is mediated by LDLR family receptors .

Recombinant RAP is widely used in laboratory research to study receptor-ligand interactions, receptor trafficking, and the molecular mechanisms underlying various cellular processes. It is particularly useful in experiments involving the LDLR family, as it can be used to block receptor-ligand interactions and study the resulting effects on cellular functions .

The recombinant protein is typically provided as a sterile, lyophilized (freeze-dried) powder. It is recommended to reconstitute the lyophilized RAP in sterile water to a concentration of at least 100 µg/ml, which can then be further diluted for use in various experimental setups . The protein is stable at room temperature for up to three weeks when lyophilized, but for long-term storage, it should be kept desiccated below -18°C. Once reconstituted, it should be stored at 4°C for short-term use (2-7 days) and below -18°C for long-term storage .