RCAN3 Human

Regulator of Calcineurin 3 Human Recombinant
Cat. No.
BT12283
Source
E.coli.
Synonyms
DSCR1L2, hRCN3, MCIP3, RCN3, Calcipressin-3, Down syndrome candidate region 1-like protein 2, Myocyte-enriched calcineurin-interacting protein 3, Regulator of calcineurin 3.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

RCAN3 Human Recombinant produced in E. coli is a single polypeptide chain containing 209 amino acids (56-241) and having a molecular mass of 23.5 kDa. RCAN3 is fused to 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Calcipressin-3 (RCAN3) is a protein involved in the development of the central nervous system. It functions by inhibiting calcineurin, a protein phosphatase, thereby suppressing calcineurin-dependent transcriptional responses. RCAN3 achieves this by binding to the catalytic domain of calcineurin A. When overexpressed, RCAN3 effectively inhibits calcineurin's activity on nuclear factor of activated T-cells (NFAT) transcription factors, leading to a downregulation of NFAT-dependent cytokine gene expression in activated Jurkat T-cells. RCAN3 is found in various tissues, with the highest expression observed in the heart, skeletal muscle, kidney, liver, and peripheral blood leukocytes.
Description
Recombinant human RCAN3, produced in E. coli, is a single polypeptide chain comprising 209 amino acids (residues 56-241) with a molecular weight of 23.5 kDa. The protein is engineered with a 23 amino acid His-tag fused at the N-terminus to facilitate purification using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The RCAN3 solution is provided at a concentration of 0.5 mg/mL in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 2 M Urea, 20% glycerol, and 0.2 M NaCl.
Stability
For short-term storage (2-4 weeks), the RCAN3 solution should be kept refrigerated at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Repeated freezing and thawing of the solution should be avoided.
Purity
The purity of RCAN3 is determined to be greater than 90% using SDS-PAGE analysis.
Synonyms
DSCR1L2, hRCN3, MCIP3, RCN3, Calcipressin-3, Down syndrome candidate region 1-like protein 2, Myocyte-enriched calcineurin-interacting protein 3, Regulator of calcineurin 3.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSEAVFEAR EQKERFEALF TIYDDQVTFQ LFKSFRRVRI NFSKPEAAAR ARIELHETDF NGQKLKLYFA QVQMSGEVRD KSYLLPPQPV KQFLISPPAS PPVGWKQSED AMPVINYDLL CAVSKLGPGE KYELHAGTES TPSVVVHVCE SETEEEEETK NPKQKIAQTR RPDPPTAALN EPQTFDCAL.

Product Science Overview

Introduction

The Regulator of Calcineurin 3 (RCAN3) is a protein that plays a crucial role in the regulation of calcineurin, a serine/threonine phosphatase involved in various cellular processes. Calcineurin is activated by increased intracellular calcium concentrations and is essential for linking calcium signaling to the phosphorylation states of numerous substrates .

Calcineurin and Its Regulation

Calcineurin is a key player in calcium signaling pathways. It is activated by the binding of calcium ions to the calcium-sensing protein calmodulin, which then binds to calcineurin to form an active phosphatase complex . This process directly couples calcium signaling to dephosphorylation, impacting various cellular functions such as transcription, muscle contraction, and immune responses .

Role of RCAN3

RCAN3, also known as DSCR1L2, is one of the regulators of calcineurin. It is part of a family of proteins that modulate calcineurin activity by binding to it and inhibiting its phosphatase activity. This regulation is crucial for maintaining cellular homeostasis and preventing overactivation of calcineurin, which can lead to pathological conditions .

Human Recombinant RCAN3

Recombinant human RCAN3 is a laboratory-produced version of the naturally occurring protein. It is used in research to study the regulatory mechanisms of calcineurin and to develop potential therapeutic interventions for diseases associated with dysregulated calcineurin activity. The recombinant protein is typically produced in bacterial or mammalian expression systems and purified for use in various experimental applications .

Applications in Research

The study of recombinant human RCAN3 has provided insights into the molecular mechanisms of calcineurin regulation. It has been used to investigate the role of calcineurin in muscle performance, immune responses, and neurological functions. For example, research has shown that variations in RCAN3 expression can influence muscle performance by modulating calcineurin signaling pathways .

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