RAPSN Mouse

Receptor-Associated Protein Of The Synapse Mouse Recombinant
Cat. No.
BT10983
Source
Escherichia Coli.
Synonyms
Receptor-Associated Protein Of The Synapse, 43 KDa Postsynaptic Protein, Acetylcholine Receptor-Associated 43 KDa Protein, RNF205, RING Finger Protein 205, rapsyn, Receptor-Associated Protein Of The Synapse, 43Kd, 43 KDa Receptor-Associated Protein Of The Synapse, RAPSYN, RAPsyn, CMS1D, CMS1E.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

RAPSN Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 435 amino acids (1-412aa) and having a molecular mass of 48.8kDa.
RAPSN is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Receptor-associated protein of the synapse (RAPSN), with a molecular weight of 43 kDa, is found in skeletal muscle's postsynaptic membrane. It is crucial for clustering nicotinic acetylcholine receptors (nAChR). RAPSN self-associates through at least two of its seven tetra-tricopeptide repeats (TPRs). It interacts with the nAChR α subunit's large intracellular domain, extending to the coiled-coil domain's hydrophobic surface. RAPSN influences AChR trafficking within the cell. Overexpression hinders the agrin-induced AChR clustering pathway. RAPSN is also involved in postsynaptic congenital myasthenic syndromes.
Description
Recombinant Mouse RAPSN, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 435 amino acids (1-412aa). It has a molecular weight of 48.8 kDa. A 23 amino acid His-tag is fused to the N-terminus of RAPSN. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
A clear solution that has been sterilized by filtration.
Formulation
RAPSN protein solution at a concentration of 0.5 mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0), 0.4M Urea, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein like 0.1% HSA or BSA is recommended for long-term storage. Repeated freezing and thawing cycles should be avoided.
Purity
Purity exceeds 85% as assessed by SDS-PAGE.
Synonyms
Receptor-Associated Protein Of The Synapse, 43 KDa Postsynaptic Protein, Acetylcholine Receptor-Associated 43 KDa Protein, RNF205, RING Finger Protein 205, rapsyn, Receptor-Associated Protein Of The Synapse, 43Kd, 43 KDa Receptor-Associated Protein Of The Synapse, RAPSYN, RAPsyn, CMS1D, CMS1E.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMGQDQTK QQIEKGLQLY QSNQTEKALQ VWMKVLEKGS DLVGRFRVLG CLVTAHSEMG RYKEMLKFAV VQIDTARGLE DADFLLESYL NLARSNEKLC EFHKTISYCK TCLGLPGTRA GAQLGGQVSL SMGNAFLGLS LFQKALESFE KALRYAHNND DTMLECRVCC SLGSFYAQVK DYEKALFFPC KAAELVNDYG KGWSLKYRAM SQYHMAVAYR LLGHLGSAME CCEESMKIAL QHGDRPLQAL CLLCFADIHR SRGDLETAFP RYDSAMSIMT EIGNRLGQVH VLLGVAKCWM ARKVQDKALD AIEKAQDLAE EVGNKLSQLK LHCLSESIYR SKGLQRELRT HVVRFHECVE ETELYCGLCG ESIGERNSRL QALPCSHIFH LRCLQNNGTR SCPNCRRSSM KPGFV

Product Science Overview

Introduction

The Receptor-Associated Protein Of The Synapse (RAPSN), also known as rapsyn, is a crucial protein involved in the neuromuscular junction. This protein is encoded by the RAPSN gene and plays a significant role in the clustering and anchoring of nicotinic acetylcholine receptors (AChRs) at synaptic sites. The mouse recombinant version of this protein is often used in research to study its function and role in various biological processes.

Structure and Function

RAPSN is a 43 kDa protein that contains a conserved cAMP-dependent protein kinase phosphorylation site . This site is essential for its function in the neuromuscular junction. The primary role of RAPSN is to link AChRs to the underlying postsynaptic cytoskeleton, which it achieves possibly through direct association with actin or spectrin . This linkage is vital for the stabilization and proper functioning of the synapse.

Role in Health and Disease

RAPSN is critical for maintaining the structure and function of the neuromuscular junction. Mutations in the RAPSN gene can lead to congenital myasthenic syndromes (CMS), a group of disorders characterized by muscle weakness and fatigue . These mutations disrupt the normal clustering of AChRs, leading to impaired synaptic transmission and muscle function.

Research and Applications

The mouse recombinant version of RAPSN is widely used in research to understand its role in synaptic function and its involvement in neuromuscular diseases. Studies using recombinant RAPSN have provided insights into the molecular mechanisms underlying synaptic stabilization and the pathogenesis of CMS .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.