RAN Human
RAN, Member RAS Oncogene Family Human Recombinant
Cat. No.
BT10383
Source
Escherichia Coli.
Synonyms
ARA24, Gsp1, TC4, GTP-binding nuclear protein Ran, GTPase Ran, Ras-related nuclear protein, Ras-like protein TC4, Androgen receptor-associated protein 24, Ras-like, RANP1.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
RAN Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 236 amino acids (1-216 a.a.) and having a molecular mass of 26.5 kDa. The RAN is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Introduction
RAN, a GTP-binding protein, plays a crucial role in nucleocytoplasmic transport by facilitating protein import into the nucleus and RNA export. It is also involved in chromatin condensation and cell cycle regulation. During mitosis, RAN interacts with BIRC5 to form a complex that acts as a scaffold, aiding in the delivery of the RAN effector molecule TPX2 to microtubules, thereby contributing to mitotic spindle formation. Furthermore, RUN enhances AR-mediated transactivation.
Description
Recombinant Human RAN, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 236 amino acids (with a sequence spanning from amino acid position 1 to 216) and possessing a molecular weight of 26.5 kDa. A 20 amino acid His-Tag is fused to the N-terminus of the RAN protein to facilitate purification, which is achieved through proprietary chromatographic techniques.
Physical Appearance
The product is a sterile, colorless solution that has been filtered for sterility.
Formulation
The provided solution of Human RAN is formulated in a buffer containing 20mM Tris-HCl at pH 8, 1mM DTT, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. It's important to avoid repeated freeze-thaw cycles to maintain product integrity.
Purity
The purity of the protein is determined to be greater than 90% using SDS-PAGE analysis.
Synonyms
ARA24, Gsp1, TC4, GTP-binding nuclear protein Ran, GTPase Ran, Ras-related nuclear protein, Ras-like protein TC4, Androgen receptor-associated protein 24, Ras-like, RANP1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MAAQGEPQVQ FKLVLVGDGG TGKTTFVKRH LTGEFEKKYV ATLGVEVHPL VFHTNRGPIK FNVWDTAGQE KFGGLRDGYY IQAQCAIIMF DVTSRVTYKN VPNWHRDLVR VCENIPIVLC GNKVDIKDRK VKAKSIVFHR KKNLQYYDIS AKSNYNFEKP FLWLARKLIG DPNLEFVAMP ALAPPEVVMD PALAAQYEHD LEVAQTTALP DEDDDL.
Product Science Overview
Structure and Function
RAN is a 25 kDa protein encoded by the RAN gene. It exists in two nucleotide-bound forms: GDP-bound (RanGDP) and GTP-bound (RanGTP). The conversion between these forms is regulated by RCC1 (Ran Guanine nucleotide Exchange Factor) and RanGAP (Ran GTPase Activating Protein) . This conversion is essential for the directionality of nucleocytoplasmic transport, which is regulated by GTP hydrolysis .
Role in Cellular Processes
- Nucleocytoplasmic Transport: RAN is essential for the translocation of RNA and proteins through the nuclear pore complex. It shuttles between the nucleus and the cytoplasm, ensuring the proper transport of macromolecules .
- DNA Synthesis and Cell Cycle Progression: RAN is involved in the control of DNA synthesis and cell cycle progression. Mutations in RAN can disrupt DNA synthesis, highlighting its importance in maintaining cellular functions .
- Microtubule Organization: RAN regulates the formation and organization of the microtubule network independently of its role in nucleocytoplasmic transport. It is a key signaling molecule that regulates microtubule polymerization during mitosis .
Clinical Significance
RAN is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy’s disease (X-linked spinal and bulbar muscular atrophy). The coactivation of AR by RAN diminishes with polyglutamine expansion, potentially leading to partial androgen insensitivity during the development of Kennedy’s disease .
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
