RALY Human

RALY Human Recombinant
Cat. No.
BT10572
Source
Escherichia Coli.
Synonyms
RALY Heterogeneous Nuclear Ribonucleoprotein, RNA-Binding Protein (Autoantigenic, HnRNP-Associated With Lethal Yellow), HnRNP Associated With Lethal Yellow Protein Homolog, Heterogeneous Nuclear Ribonucleoprotein C-Like 2, HnRNP Core Protein C-Like 2, Autoantigen P542, HNRPCL2, P542, RNA Binding Protein, Autoantigenic (HnRNP-Associated With Lethal Yellow Homolog (Mouse)), RNA Binding Protein, Autoantigenic (HnRNP-Associated With Lethal Yellow Homolog), RNA-Binding Protein (Autoantigenic), RNA-Binding Protein Raly, RNA-binding protein Raly.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

RALY Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 329 amino acids (1-306 a.a) and having a molecular mass of 34.9kDa.
RALY is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
RALY, a member of the heterogeneous nuclear ribonucleoprotein (hnRNP) gene family, plays a crucial role in pre-mRNA splicing and embryonic development. Notably, alternative splicing of RALY results in multiple transcript variants.
Description
Recombinant Human RALY, expressed in E. coli, is a non-glycosylated polypeptide chain comprising 329 amino acids (1-306 a.a) with a molecular weight of 34.9 kDa. The protein is engineered with a 23 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The RALY protein solution is supplied at a concentration of 0.5 mg/ml in a buffer containing 20 mM Tris-HCl (pH 8.0) and 10% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended periods, store frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Repeated freezing and thawing cycles should be avoided.
Purity
The purity of the protein is greater than 90.0% as determined by SDS-PAGE analysis.
Synonyms
RALY Heterogeneous Nuclear Ribonucleoprotein, RNA-Binding Protein (Autoantigenic, HnRNP-Associated With Lethal Yellow), HnRNP Associated With Lethal Yellow Protein Homolog, Heterogeneous Nuclear Ribonucleoprotein C-Like 2, HnRNP Core Protein C-Like 2, Autoantigen P542, HNRPCL2, P542, RNA Binding Protein, Autoantigenic (HnRNP-Associated With Lethal Yellow Homolog (Mouse)), RNA Binding Protein, Autoantigenic (HnRNP-Associated With Lethal Yellow Homolog), RNA-Binding Protein (Autoantigenic), RNA-Binding Protein Raly, RNA-binding protein Raly.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMSLKLQA SNVTNKNDPK SINSRVFIGN LNTALVKKSD VETIFSKYGR VAGCSVHKGY AFVQYSNERH ARAAVLGENG RVLAGQTLDI NMAGEPKPDR PKGLKRAASA IYSGYIFDYD YYRDDFYDRL FDYRGRLSPV PVPRAVPVKR PRVTVPLVRR VKTNVPVKLF ARSTAVTTSS AKIKLKSSEL QAIKTELTQI KSNIDALLSR LEQIAAEQKA NPDGKKKGDG GGAGGGGGGG GSGGGGSGGG GGGGSSRPPA PQENTTSEAG LPQGEARTRD DGDEEGLLTH SEEELEHSQD TDADDGALQ.

Product Science Overview

Introduction

RALY, also known as RNA-binding protein Raly, is a member of the heterogeneous nuclear ribonucleoprotein (hnRNP) family. This protein plays a crucial role in pre-mRNA splicing and embryonic development . The human recombinant form of RALY is produced using Escherichia coli (E. coli) and is often used in laboratory research.

Gene and Protein Structure

The RALY gene is located on chromosome 20q11.22 . The protein encoded by this gene consists of 329 amino acids and has a molecular mass of approximately 34.9 kDa . The recombinant form of RALY is fused to a 23 amino acid His-tag at the N-terminus, which facilitates its purification through chromatographic techniques .

Function and Mechanism

RALY is involved in the processing of pre-mRNA, a critical step in the gene expression pathway. It binds to RNA and participates in the splicing process, which removes introns from pre-mRNA transcripts . This function is essential for the proper expression of genes and the production of functional proteins.

Additionally, RALY has been implicated in embryonic development. Its role in this process is not fully understood, but it is believed to be involved in the regulation of gene expression during early development .

Expression and Localization

RALY is ubiquitously expressed in various human tissues, with higher expression levels observed in certain cell lines . Immunofluorescence studies have shown that RALY predominantly localizes to the nucleus and perinuclear regions of cells .

Recombinant Production

The recombinant form of RALY is produced in E. coli as a single, non-glycosylated polypeptide chain . The protein is purified using proprietary chromatographic techniques to achieve a purity greater than 90% as determined by SDS-PAGE . The recombinant RALY protein is typically supplied in a sterile, filtered, colorless solution containing Tris-HCl buffer and glycerol .

Applications

Recombinant RALY is primarily used in laboratory research to study its function and role in various biological processes. It is also used in experiments to understand its involvement in diseases and to develop potential therapeutic interventions.

Storage and Stability

The recombinant RALY protein should be stored at 4°C if it is to be used within 2-4 weeks. For longer storage periods, it should be frozen at -20°C. To ensure stability, it is recommended to add a carrier protein such as human serum albumin (HSA) or bovine serum albumin (BSA) and to avoid multiple freeze-thaw cycles .

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