RAGE Human, Sf9
Sf9, Baculovirus cells.
Advanced Glycosylation End-Product Specific Receptor, Advanced Glycosylation End Product-Specific Receptor, RAGE, Receptor for Advanced Glycation End-Products Variant 20, Receptor for Advanced Glycosylation End Products, Receptor for Advanced Glycation End-Products, RAGE Isoform NtRAGE-Delta, RAGE Isoform SRAGE-Delta, SCARJ1.
Greater than 90.0% as determined by SDS-PAGE.
Description
RAGE Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 561 amino acids (24-342 a.a) and having a molecular mass of 61.2kDa. RAGE is fused to a 242 amino acid hIgG-His-tag at C-terminus & purified by proprietary chromatographic techniques.
Product Specs
Advanced Glycosylation End-Product Specific Receptor, Advanced Glycosylation End Product-Specific Receptor, RAGE, Receptor for Advanced Glycation End-Products Variant 20, Receptor for Advanced Glycosylation End Products, Receptor for Advanced Glycation End-Products, RAGE Isoform NtRAGE-Delta, RAGE Isoform SRAGE-Delta, SCARJ1.
Sf9, Baculovirus cells.
ADLQNITARI GEPLVLKCKG APKKPPQRLE WKLNTGRTEA WKVLSPQGGG PWDSVARVLP NGSLFLPAVG IQDEGIFRCQ AMNRNGKETK SNYRVRVYQI PGKPEIVDSA SELTAGVPNK VGTCVSEGSY PAGTLSWHLD GKPLVPNEKG VSVKEQTRRH PETGLFTLQS ELMVTPARGG DPRPTFSCSF SPGLPRHRAL RTAPIQPRVW EPVPLEEVQL VVEPEGGAVA PGGTVTLTCE VPAQPSPQIH WMKDGVPLPL PPSPVLILPE IGPQDQGTYS CVATHSSHGP QESRAVSISI IEPGEEGPTA GSVGGSGLGT LALEPKSCDK THTCPPCPAP ELLGGPSVFL FPPKPKDTLM ISRTPEVTCV VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK VSNKALPAPI EKTISKAKGQ PREPQVYTLP PSRDELTKNQ VSLTCLVKGF YPSDIAVEWE SNGQPENNYK TTPPVLDSDG SFFLYSKLTV DKSRWQQGNV FSCSVMHEA HNHYTQKSLS LSPGKHHHHH H.
Product Science Overview
The Advanced Glycosylation End Product-Specific Receptor (AGER), commonly known as the Receptor for Advanced Glycation End Products (RAGE), is a member of the immunoglobulin superfamily of cell surface receptors. This receptor plays a crucial role in various physiological and pathological processes, including inflammation, development, and homeostasis .
RAGE is a 35 kilodalton transmembrane receptor that was first characterized in 1992. It is known for its ability to bind advanced glycation end products (AGEs), which are nonenzymatically glycosylated proteins that accumulate in vascular tissues during aging and at an accelerated rate in diabetes . The receptor has a broad ligand repertoire, including AGEs, S100 proteins, high-mobility group box 1 protein (HMGB1), amyloid beta, nucleic acids, phospholipids, and glycosaminoglycans .
RAGE is implicated in the pathogenesis of various diseases, such as diabetes, Alzheimer’s disease, vascular complications, neurodegenerative disorders, and cancers. The interaction between RAGE and its ligands results in the activation of pro-inflammatory gene expression, contributing to the progression of these diseases .
The human recombinant form of RAGE is often expressed in Sf9 cells, which are derived from the fall armyworm Spodoptera frugiperda. Sf9 cells are commonly used in the baculovirus expression system, which is a powerful tool for producing recombinant proteins. This system allows for high-level expression and proper post-translational modifications, making it suitable for producing functional human proteins .
