RABIF Human
Description
RABIF is fused to a 20 amino acid His Tag at N-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
RAB Interacting Factor (RABIF), also known as MSS4 (Mammalian Suppressor of SEC4), is a protein encoded by the RABIF gene in humans. This protein plays a crucial role in intracellular vesicular transport, which is essential for various cellular processes, including secretion, endocytosis, and membrane recycling.
The RABIF gene is located on chromosome 1 and encodes a protein that belongs to the SEC4/YPT1/RAB family of small GTP-binding proteins. These proteins are involved in the regulation of intracellular vesicular transport. The RABIF protein is composed of several domains that facilitate its interaction with other proteins and its function as a guanine-nucleotide exchange factor (GEF).
RABIF functions as a guanine-nucleotide-releasing protein that acts on members of the SEC4/YPT1/RAB subfamily. It stimulates the release of GDP (guanosine diphosphate) from these proteins, allowing them to bind GTP (guanosine triphosphate) and become active. Specifically, RABIF stimulates GDP release from YPT1, RAB3A, and RAB10, although it is less active on these proteins compared to the SEC4 protein .
The primary role of RABIF is to facilitate vesicular transport within the cell. Vesicular transport is a critical process that involves the movement of vesicles, which are small membrane-bound sacs, between different cellular compartments. This process is essential for the proper functioning of various cellular activities, including the transport of proteins and lipids, signal transduction, and the maintenance of cellular homeostasis.
Mutations or dysregulation of the RABIF gene can lead to various diseases. For example, RABIF has been associated with autoimmune cholangitis and vulvar liposarcoma . Additionally, its involvement in vesicular transport suggests that it may play a role in other diseases related to cellular transport and signaling.
Recombinant RAB Interacting Factor (Human) is a laboratory-produced version of the human RABIF protein. It is typically used in research to study the function of the RABIF protein and its role in vesicular transport. The recombinant protein is produced using recombinant DNA technology, which involves inserting the RABIF gene into a suitable expression system, such as bacteria or yeast, to produce the protein in large quantities.
The recombinant RABIF protein is often used in various biochemical assays to study its interaction with other proteins, its GEF activity, and its role in cellular processes. It is also used in structural studies to determine the three-dimensional structure of the protein, which can provide insights into its function and mechanism of action.
