RAB8 Human
RAB8, Member RAS Oncogene Family Human Recombinant
Cat. No.
BT10256
Source
Escherichia Coli.
Synonyms
Ras-related protein Rab-8B, RAB8B.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
RAB8 Human Recombinant produced in E. coli is a single polypeptide chain containing 227 amino acids (1-204) and having a molecular mass of 25.7kDa. RAB8 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
RAB8, a member of the RAS oncogene family (RAB8B), is a small GTPase found on the surfaces of membrane-bound organelles within the cytoplasm. It plays a crucial role in various cellular processes, including the targeted transport of vesicles, the release of neurotransmitters, and the regulation of cell junction dynamics in Sertoli cells. RAB proteins, including RAB8, facilitate intracellular vesicle transport by enabling the precise docking and fusion of vesicles with their intended target membranes.
Description
Recombinant human RAB8, produced in E. coli, is a single polypeptide chain consisting of 227 amino acids (residues 1-204) with a molecular weight of 25.7 kDa. This protein is engineered with a 23 amino acid His-tag at the N-terminus to facilitate purification using proprietary chromatographic methods.
Physical Appearance
A clear solution that has undergone sterile filtration.
Formulation
The RAB8 solution is provided at a concentration of 1 mg/ml and contains 20 mM Tris-HCl buffer (pH 8.0), 0.4 M urea, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), keep the solution refrigerated at 4°C. For longer storage, freeze the solution at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for extended storage. Repeated freezing and thawing of the solution should be avoided.
Purity
Purity exceeds 90% as determined by SDS-PAGE analysis.
Synonyms
Ras-related protein Rab-8B, RAB8B.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAKTYDY LFKLLLIGDS GVGKTCLLFR FSEDAFNTTF ISTIGIDFKI RTIELDGKKI KLQIWDTAGQ ERFRTITTAY YRGAMGIMLV YDITNEKSFD NIKNWIRNIE EHASSDVERM ILGNKCDMND KRQVSKERGE KLAIDYGIKF LETSAKSSAN VEEAFFTLAR DIMTKLNRKM NDSNSAGAGG PVKITENRSK KTSFFRC.
Product Science Overview
Introduction
Gene and Protein Structure
Function
RAB8A is involved in various cellular processes, including vesicle formation, movement, tethering, and fusion. It cycles between an inactive GDP-bound form and an active GTP-bound form. In its active form, RAB8A recruits different sets of downstream effectors that are directly responsible for vesicle formation and trafficking .
Pathways and Interactions
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