RAB7A Antibody

RAB7A, a member of the RAS oncogene family, is a small GTPase that plays a crucial role in the regulation of vesicular transport. It is part of the RAB family of proteins, which are involved in various cellular processes, including endocytosis, exocytosis, and vesicle trafficking . The RAB7A gene is located on chromosome 3 and encodes the RAB7A protein, which is essential for the late endosomal and lysosomal transport .

RAB7A is a small GTP-binding protein that cycles between an active GTP-bound state and an inactive GDP-bound state. In its active state, RAB7A interacts with various effector proteins to regulate endo-lysosomal trafficking . This protein is involved in early-to-late endosomal maturation, endosome-lysosome transport, and the positioning of endosomes along microtubules .

RAB7A is widely expressed in various tissues, including skeletal muscle, neurons, osteoclasts, and macrophages . Its expression is crucial for the proper functioning of these cells, particularly in processes involving vesicle trafficking and lysosomal degradation .

The primary function of RAB7A is to regulate vesicle trafficking from early to late endosomes and to lysosomes . It plays a central role in endosomal maturation, endosome-lysosome transport, and the positioning of endosomes along microtubules . Additionally, RAB7A is involved in various cellular and physiological events, such as growth-factor-mediated cell signaling, nutrient uptake, neurotrophin transport in neurons, and lipid metabolism .

RAB7A functions by cycling between its active GTP-bound state and inactive GDP-bound state . In its active state, it binds to effector proteins that facilitate vesicle trafficking and endosomal maturation . This cycling is regulated by guanine nucleotide exchange factors (GEFs) that promote the exchange of GDP for GTP, and GTPase-activating proteins (GAPs) that stimulate the hydrolysis of GTP to GDP .

The activity of RAB7A is regulated by its interaction with GEFs and GAPs . GEFs activate RAB7A by promoting the exchange of GDP for GTP, while GAPs inactivate RAB7A by stimulating the hydrolysis of GTP to GDP . Additionally, post-translational modifications, such as phosphorylation, can also regulate the activity and function of RAB7A .

The Mouse Anti Human RAB7A antibody is a monoclonal antibody that specifically targets the RAB7A protein . This antibody is commonly used in research to study the expression, localization, and function of RAB7A in various cellular processes . It is also used in various applications, including Western blotting, immunocytochemistry, and flow cytometry .