RAB5A Antibody

RAB5A is a member of the Ras superfamily of small GTPases, which are involved in various cellular processes, including vesicular trafficking. The RAB5A protein is localized at the plasma membrane and early endosomes, playing a crucial role in the regulation of endocytosis. The mouse anti-human RAB5A antibody is a monoclonal antibody produced by immunizing mice with a synthetic peptide corresponding to residues surrounding Glycine 190 of the human RAB5A protein .

RAB5A is a key regulator of vesicular trafficking during early endocytosis. It functions by cycling between an active GTP-bound state and an inactive GDP-bound state. This conformational change is essential for its biological function as a rate-limiting regulator at multiple steps during endocytosis . RAB5A interacts with several specific effectors to form complexes on a specialized RAB domain of the endosome, promoting the recycling of cargo between the endosome and the plasma membrane .

The mouse anti-human RAB5A antibody is produced by immunizing mice with a synthetic peptide corresponding to residues surrounding Glycine 190 of the human RAB5A protein. The monoclonal antibody is then purified using protein G affinity chromatography to ensure high specificity and purity .

The mouse anti-human RAB5A antibody can be used in various scientific applications, including:

• Western Blotting (WB): Detects endogenous levels of total RAB5A protein.
• Immunoprecipitation (IP): Used to isolate RAB5A protein from cell extracts.
• Immunohistochemistry (IHC): Detects RAB5A in paraffin-embedded tissue sections.
• Immunofluorescence (IF): Used to visualize RAB5A in fixed cells .

The antibody is supplied in a buffer containing 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol, and less than 0.02% sodium azide. It should be stored at -20°C and should not be aliquoted to maintain its stability .