RAB3IL1 Human

RAB3A Interacting Protein Like 1 Human Recombinant
Cat. No.
BT9466
Source
Escherichia Coli.
Synonyms
GRAB, Rab-3A-interacting-like protein 1, Guanine nucleotide exchange factor for Rab-3A, Rab3A-interacting-like protein 1, Rabin3-like 1.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

RAB3IL1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 405 amino acids (1-382a.a) and having a molecular mass of 45kDa.
RAB3IL1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
RAB3A Interacting Protein Like 1 (RAB3IL1) is a protein comprising 382 amino acids. It belongs to the SEC2 family and functions as a guanine nucleotide exchange factor (GEF) for Rab3A. Notably, RAB3IL1 engages in competition with IHPK1 to interact with Rab3A through its coiled coil domain. This competitive interaction is independent of IHPK1's kinase activity.
Description
RAB3IL1 Human Recombinant, generated using E. coli, is an unglycosylated polypeptide chain. It consists of 405 amino acids (1-382a.a) with a molecular weight of 45kDa. The protein includes a 23 amino acid His-tag at the N-terminus. Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
A clear solution that has undergone sterile filtration.
Formulation
RAB3IL1 protein solution at a concentration of 0.25mg/ml. This solution contains phosphate buffer saline with a pH of 7.4 and 50% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be kept at 4°C. For extended storage, it should be stored frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. It is crucial to avoid repeated freezing and thawing cycles.
Purity
SDS-PAGE analysis indicates a purity exceeding 90.0%.
Synonyms
GRAB, Rab-3A-interacting-like protein 1, Guanine nucleotide exchange factor for Rab-3A, Rab3A-interacting-like protein 1, Rabin3-like 1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMWSGPPQ PDQGLPPPLA AVPVPWKSTD PCQGHRESPG ALVETSAGEE AQGQEGPAAA QLDVLRLRSS SMEIREKGSE FLKEELHRAQ KELKLKDEEC ERLSKVREQL EQELEELTAS LFEEAHKMVR EANMKQAASE KQLKEARGKI DMLQAEVTAL KTLVITSTPA SPNRELHPQL LSPTKAGPRK GHSRHKSTSS TLCPAVCPAA GHTLTPDREG KEVDTILFAE FQAWRESPTL DKTCPFLERV YREDVGPCLD FTMQELSVLV RAAVEDNTLT IEPVASQTLP TVKVAEVDCS STNTCALSGL TRTCRHRIRL GDSKSHYYIS PSSRARITAV CNFFTYIRYI QQGLVRQDAE PMFWEIMRLR KEMSLAKLGF FPQEA.

Product Science Overview

Introduction

RAB3A Interacting Protein Like 1 (RAB3IL1), also known as Guanine Nucleotide Exchange Factor for Rab-3A (GRAB), is a protein encoded by the RAB3IL1 gene in humans. This protein is a member of the SEC2 family and plays a crucial role in the regulation of synaptic vesicle exocytosis by activating Rab3A, a GTPase .

Function and Mechanism

RAB3IL1 functions as a guanine nucleotide exchange factor (GEF) for Rab3A. It promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form . This activation is essential for the regulation of vesicle-mediated transport and Rab regulation of trafficking . Additionally, RAB3IL1 may also activate other Rab proteins such as RAB8A and RAB8B .

Expression and Localization

RAB3IL1 is expressed in various tissues, including lymphoid tissue, bone marrow, testis, and skeletal muscle . It is involved in several biological processes such as protein ubiquitination, immune response, and spermatid development . The protein interacts with multiple other proteins, indicating its involvement in complex cellular pathways .

Clinical Significance

Mutations or dysregulation of the RAB3IL1 gene have been associated with certain diseases, including Spinal Muscular Atrophy with Lower Extremity Predominant 1 . Understanding the function and regulation of RAB3IL1 can provide insights into the molecular mechanisms underlying these conditions and potentially lead to the development of targeted therapies.

Research and Applications

Human recombinant RAB3IL1 is used in various research applications to study its role in cellular processes and disease mechanisms. The recombinant protein is produced using advanced biotechnological methods to ensure high purity and activity . Researchers utilize this protein to investigate its interactions, regulatory mechanisms, and potential therapeutic applications.

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