RAB3B, a member of the RAS oncogene family, is a protein-coding gene that plays a crucial role in various cellular processes. This gene is particularly significant in the context of synaptic transmission and vesicular trafficking. The human recombinant form of RAB3B is produced using recombinant DNA technology, which allows for the expression of the protein in a controlled environment, typically in bacterial or mammalian cells.
The RAB3B gene is located on chromosome 1 and encodes a protein that is part of the RAB family of small GTPases. These proteins are involved in the regulation of vesicle trafficking, which is essential for the proper functioning of cellular processes. The RAB3B protein consists of 239 amino acids and has a molecular mass of approximately 26.9 kDa . It is characterized by its ability to bind GDP and GTP, which are critical for its function as a molecular switch in vesicle trafficking.
RAB3B is primarily involved in the regulation of synaptic vesicle cycle and vesicle size. It plays a significant role in the positive regulation of dopamine uptake involved in synaptic transmission . The protein is located in the perinuclear region of the cytoplasm and is an anchored component of the synaptic vesicle membrane. By binding to myosin V, RAB3B facilitates the transport of vesicles within the cell, ensuring that neurotransmitters are efficiently released at synaptic junctions.
The human recombinant form of RAB3B is produced in E. coli as a single, non-glycosylated polypeptide chain . The recombinant protein is fused to a 20 amino acid His-Tag at the N-terminus, which facilitates its purification using chromatographic techniques. The protein is supplied as a sterile filtered clear solution and is formulated in a buffer containing Tris-HCl, NaCl, DTT, and glycerol . This recombinant form is used in various research applications, including studies on vesicle trafficking, synaptic transmission, and potential therapeutic interventions.