RAB32 Human

RAB32, Member RAS Oncogene Family Human Recombinant
Cat. No.
BT8902
Source
E.coli.
Synonyms
RAB32 member RAS oncogene family, ras-related protein Rab-32.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

RAB32 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 249 amino acids (1-225) and having a molecular mass of 27.6 kDa.
RAB32 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
RAB32, a member of the small GTPase superfamily, plays a crucial role in regulating ER calcium levels and modifying calnexin's enhancement on the mitochondria-associated membrane (MAM) without impacting the ER distribution of protein-disulfide isomerase and mitofusin-2. Additionally, RAB32 influences the localization of cAMP-dependent protein kinase (PKA) to mitochondrial and ER membranes. Its inactivation or overexpression affects the phosphorylation of Drp1 and Bad. Through its dual functions in regulating MAM properties and anchoring PKA, RAB32's activity and expression levels significantly impact the rate of apoptosis initiation.
Description
Recombinant human RAB32, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 249 amino acids (1-225). It has a molecular weight of 27.6 kDa. The protein is designed with a 24 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The RAB32 solution is provided at a concentration of 0.5 mg/ml. It is formulated in a buffer consisting of 20mM Tris-HCl (pH 8.0), 5mM DTT, 0.2M NaCl, 0.2M EDTA, and 50% glycerol.
Stability
For short-term storage (2-4 weeks), keep the RAB32 vial refrigerated at 4°C. For extended storage, freeze the solution at -20°C. Adding a carrier protein such as HSA or BSA (0.1%) is recommended for long-term storage. To maintain protein integrity, avoid repeated freeze-thaw cycles.
Purity
The purity of RAB32 is determined to be greater than 90% using SDS-PAGE analysis.
Synonyms
RAB32 member RAS oncogene family, ras-related protein Rab-32.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMAGGGA GDPGLGAAAA PAPETREHLF KVLVIGELGV GKTSIIKRYV HQLFSQHYRA TIGVDFALKV LNWDSRTLVR LQLWDIAGQE RFGNMTRVYY KEAVGAFVVF DISRSSTFEA VLKWKSDLDS KVHLPNGSPI PAVLLANKCD QNKDSSQSPS QVDQFCKEHG FAGWFETSAK DNINIEEAAR FLVEKILVNH QSFPNEENDV DKIKLDQETL RAENKSQCC

Product Science Overview

Introduction

RAB32 is a member of the RAS oncogene family, which is a group of genes that encode small GTPases involved in various cellular processes. The RAB32 gene is responsible for encoding a protein that plays a crucial role in intracellular trafficking, mitochondrial dynamics, and cellular signaling pathways.

Gene and Protein Structure

The RAB32 gene is located on chromosome 6q24.3 and is a protein-coding gene . The protein encoded by RAB32 belongs to the small GTPase superfamily, which is characterized by their ability to bind and hydrolyze GTP. The RAB32 protein consists of 225 amino acids and has a molecular weight of approximately 24.8 kDa .

Functions

RAB32 has several important functions within the cell:

  1. Mitochondrial Dynamics: RAB32 acts as an A-kinase anchoring protein by binding to the type II regulatory subunit of protein kinase A and anchoring it to the mitochondrion. This interaction is crucial for the synchronization of mitochondrial fission .

  2. Autophagy and Melanosome Secretion: RAB32 is involved in the process of autophagy, where it plays a role in the maturation of phagosomes that engulf pathogens such as Staphylococcus aureus and Mycobacterium tuberculosis . Additionally, RAB32 is important for melanosome biogenesis and the control of melanin production .

  3. ER Calcium Management: RAB32 controls endoplasmic reticulum (ER) calcium management and affects the specific enhancement of calnexin on the mitochondria-associated membrane (MAM), without impacting the ER distribution of protein-disulfide isomerase and mitofusin-2 .

Clinical Significance

Variations in the RAB32 gene have been associated with certain diseases:

  • Leprosy: Genetic variations in RAB32 may be linked to susceptibility to leprosy .
  • Amelogenesis Imperfecta: RAB32 is associated with amelogenesis imperfecta, hypomaturation type, Iia4, a condition affecting the development of tooth enamel .
Recombinant RAB32

Human recombinant RAB32 is produced using recombinant DNA technology, where the RAB32 gene is cloned and expressed in a suitable host system, such as HEK293T cells . The recombinant protein is then purified and used for various research applications, including studies on protein-protein interactions, cellular signaling pathways, and disease mechanisms.

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RAB31 Human
RAB31, Member RAS Oncogene Family Recombinant Human
Cat. No.
BT8804
Source
Escherichia Coli.
THE BIOTEK
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