RAB23 Human
RAB23, Member RAS Oncogene Family Human Recombinant
Cat. No.
BT7652
Source
E.coli.
Synonyms
RAB23 member RAS oncogene family, HSPC137, RAB family small GTP binding protein RAB 23, ras-related protein Rab-23.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
RAB23 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 254 amino acids (1-234) and having a molecular mass of 28.5 kDa.
RAB23 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
RAB23 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
RAB23, a member of the Rab protein family, resides on the cytoplasmic face of the cell membrane. It participates in intracellular protein trafficking and signal transduction processes mediated by small GTPases. Importantly, RAB23 acts as a crucial negative regulator of the Sonic hedgehog signaling pathway. Mutations in the gene responsible for encoding RAB23 can lead to Carpenter syndrome, a disorder characterized by obesity, cardiac abnormalities, polysyndactyly, and craniosynostosis.
Description
Recombinant human RAB23, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 254 amino acids (residues 1-234) with a molecular weight of 28.5 kDa. This protein is fused to a 20 amino acid His-tag at the N-terminus and purified using proprietary chromatographic methods.
Physical Appearance
Clear, colorless solution that has undergone sterile filtration.
Formulation
The RAB23 solution (0.5 mg/mL) is supplied in a buffer containing 20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 mM PMSF, and 30% glycerol.
Stability
For short-term storage (2-4 weeks), keep refrigerated at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 90% as assessed by SDS-PAGE.
Synonyms
RAB23 member RAS oncogene family, HSPC137, RAB family small GTP binding protein RAB 23, ras-related protein Rab-23.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MLEEDMEVAI KMVVVGNGAV GKSSMIQRYC KGIFTKDYKK TIGVDFLERQ IQVNDEDVRL MLWDTAGQEE FDAITKAYYR GAQACVLVFS TTDRESFEAV SSWREKVVAE VGDIPTVLVQ NKIDLLDDSC IKNEEAEALA KRLKLRFYRT SVKEDLNVNE VFKYLAEKYL QKLKQQIAED PELTHSSSNK IGVFNTSGGS HSGQNSGTLN GGDVINLRPN KQRTKKNRNP FSSC
Product Science Overview
Gene and Protein Information
Structure and Expression
Function
RAB23 is involved in various cellular functions:
- Intracellular Membrane Trafficking: It regulates the movement of vesicles within cells, ensuring proper transport and fusion with target membranes .
- Autophagy and Immune Response: RAB23 plays a role in autophagy and the immune response to bacterial infections .
- Central Nervous System Development: It antagonizes sonic hedgehog signaling, which is crucial for the development of the central nervous system .
- Regulation of GLI Proteins: Together with SUFU, RAB23 prevents the nuclear import of GLI1, inhibiting its transcription factor activity. It also regulates GLI3 proteolytic processing and modulates GLI2 and GLI3 transcription factor activity .
Clinical Significance
Mutations or disruptions in the RAB23 gene have been implicated in several disorders:
Research and Applications
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