RAB17 Human

RAB17, Member RAS Oncogene Family Human Recombinant
Cat. No.
BT7093
Source
Escherichia Coli.
Synonyms
RAB17 member RAS oncogene family, ras-related protein Rab-17, FLJ12538.
Appearance
RAB17 is supplied as a sterile filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

RAB17 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 232 amino acids (1-212) and having a molecular mass of 25.6 kDa.
The RAB17 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
As a member of the small GTPase superfamily, RAB17 plays a crucial role in intracellular trafficking. It is localized in various intracellular compartments and is particularly important for regulating traffic through the apical recycling endosome. This function suggests that RAB17 is involved in the establishment and maintenance of cell polarity in epithelial cells.
Description
This product consists of a recombinant human RAB17 protein produced in E. coli. It is a single, non-glycosylated polypeptide chain comprising 232 amino acids (residues 1-212) with a molecular weight of 25.6 kDa. The protein is designed with a 20 amino acid His-Tag fused at the N-terminus to facilitate purification, which is carried out using proprietary chromatographic techniques.
Physical Appearance
The RAB17 protein is provided as a clear solution that has undergone sterile filtration.
Formulation
The RAB17 protein is supplied at a concentration of 0.5 mg/ml in a buffer consisting of 20mM Tris-HCL (pH 8), 0.1M NaCl, 1mM DTT, and 30% Glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure optimal stability during long-term storage, adding a carrier protein such as 0.1% HSA or BSA is advised. It is important to avoid repeated freeze-thaw cycles to maintain product integrity.
Purity
The purity of the RAB17 protein is greater than 90.0% as assessed by SDS-PAGE analysis.
Synonyms
RAB17 member RAS oncogene family, ras-related protein Rab-17, FLJ12538.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MAQAHRTPQP RAAPSQPRVF KLVLLGSGSV GKSSLALRYV KNDFKSILPT VGCAFFTKVV DVGATSLKLE IWDTAGQEKY HSVCHLYFRG ANAALLVYDI TRKDSFLKAQ QWLKDLEEEL HPGEVLVMLV GNKTDLSQER EVTFQEGKEF ADSQKLLFME
TSAKLNHQVS EVFNTVAQEL LQRSDEEGQA LRGDAAVALN KGPARQAKCC AH

Product Science Overview

Gene and Protein Information
  • Gene Symbol: RAB17
  • Protein Name: Ras-related protein Rab-17
  • Gene ID: 64284
  • UniProtKB/Swiss-Prot ID: Q9H0T7

RAB17 is a protein-coding gene associated with several pathways, including the metabolism of proteins and Sertoli-Sertoli cell junction dynamics. The gene is located on chromosome 2 and has several aliases, such as RAB17_HUMAN .

Function and Mechanism

RAB17 cycles between an inactive GDP-bound form and an active GTP-bound form. In its active state, it recruits various downstream effectors responsible for vesicle formation, movement, tethering, and fusion. This cycling is essential for the regulation of membrane trafficking .

One of the primary functions of RAB17 is in transcytosis, which is the directed movement of endocytosed material through the cell and its exocytosis from the plasma membrane at the opposite side. This process is mainly observed in epithelial cells and is crucial for the transcellular transport of immunoglobulins from the basolateral surface to the apical surface .

Additionally, RAB17 is required for melanosome transport and release from melanocytes. It also plays a role in the development of dendrites and dendritic spines, which are essential for neuronal connectivity and function .

Clinical Significance

Mutations or dysregulation of RAB17 have been associated with certain diseases, including Griscelli Syndrome, Type 2. This syndrome is characterized by pigmentary dilution of the skin and hair, along with immunodeficiency .

Recombinant RAB17

Recombinant RAB17 is produced using various biotechnological methods to study its function and potential therapeutic applications. Human recombinant RAB17 is available for research purposes and is used in various assays and experiments to understand its role in cellular processes .

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