RAB13 Human

RAB13, Member RAS Oncogene Family Human Recombinant

Cat. No.

BT6933

Source

Escherichia Coli.

Synonyms

RAB13 member RAS oncogene family, Cell growth-inhibiting gene 4 protein, Growth-inhibiting gene 4 protein, RAS-associated protein RAB13.

Appearance

RAB13 is supplied as a sterile filtered clear solution.

Purity

Greater than 85.0% as determined by SDS-PAGE.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

RAB13 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 220 amino acids (1-200) and having a molecular mass of 24.7 kDa.
The RAB13 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction

RAB13, a member of the small GTPase superfamily, is a non-transforming monomeric GTP-binding protein. It plays a crucial role in endocytic pathways and regulates the formation of functional epithelial tight junctions (TJs). RAB13 is expressed throughout all stages of preimplantation development and possesses four highly conserved regions involved in GTP binding and hydrolysis.

Description

Recombinant human RAB13, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 220 amino acids (1-200) with a molecular weight of 24.7 kDa. It features a 20 amino acid His-Tag at the N-terminus and is purified using proprietary chromatographic techniques.

Physical Appearance

RAB13 is provided as a clear, sterile, and filtered solution.

Formulation

RAB13 protein solution (0.25 mg/ml) is supplied in a buffer containing 20mM Tris-HCL (pH 8), 1mM DTT, 200mM NaCl, 1mM EDTA, and 20% Glycerol.

Stability

For short-term storage (2-4 weeks), store at 4°C. For extended periods, store frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.

Purity

Purity exceeds 85.0% as determined by SDS-PAGE analysis.

Synonyms

RAB13 member RAS oncogene family, Cell growth-inhibiting gene 4 protein, Growth-inhibiting gene 4 protein, RAS-associated protein RAB13.

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MAKAYDHLFK LLLIGDSGVG KTCLIIRFAE DNFNNTYIST IGIDFKIRTV DIEGKKIKLQ VWDTAGQERF KTITTAYYRG AMGIILVYDI TDEKSFENIQ NWMKSIKENA SAGVERLLLG NKCDMEAKRK VQKEQADKLA REHGIRFFET SAKSSMNVDE AFSSLARDIL LKSGGRRSGN GNKPPSTDLK TCDKKNTNKC

Product Science Overview

Gene and Protein Information

The RAB13 gene encodes a protein that is involved in the assembly of tight junctions, which are components of the apical junctional complex (AJC) of epithelial cells. The AJC is essential for forming a barrier between luminal contents and the underlying tissue. Additionally, RAB13 is involved in the endocytic recycling of occludin, regulation of epithelial cell scattering, neuronal regeneration, and regulation of neurite outgrowth.

Function and Mechanism

RAB13 cycles between an inactive GDP-bound form and an active GTP-bound form. In its active form, it recruits different sets of downstream effectors directly responsible for vesicle formation, movement, tethering, and fusion. This protein is particularly important in endocytic recycling and regulates the transport of transmembrane proteins like the tight junction protein occludin to the plasma membrane.

Clinical Significance

Mutations or dysregulation of RAB13 have been associated with various diseases, including Cardiofaciocutaneous Syndrome. The protein’s role in tight junction assembly and maintenance makes it a critical factor in maintaining epithelial cell integrity and function.

Research and Applications

RAB13 is a subject of ongoing research, particularly in the context of its role in membrane trafficking and tight junction assembly. It is also being studied as a potential biomarker for early detection of certain cancers.

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RAB13 Human

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