RSPO1 Human
CHO cells.
R-spondin-1, Cristin-3, mCristin-3, Roof plate-specific spondin-1, Rspo1, RSPO, R-spondin 1, R-spondin, Rspondin, CRISTIN3.
Greater than 95.0% as determined by SDS-PAGE.
Description
R-Spondin-1 Human Recombinant produced in CHO cells is a glycosylated monomer chain containing 243 amino acids and having a total molecular mass of 25.6kDa.
RSPO1 is purified by proprietary chromatographic techniques.
Product Specs
R-Spondin-1 (Rspo1), a member of the Rspondin family, functions as an activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Rspo1 plays a crucial role in initiating crypt cell proliferation and enhancing intestinal epithelial healing. It also negatively regulates the TGF-beta pathway.
Recombinant Human R-Spondin-1, expressed in CHO cells, is a glycosylated monomeric chain comprising 243 amino acids, resulting in a total molecular mass of 25.6kDa.
The purification of RSPO1 is carried out using proprietary chromatographic techniques.
The protein solution undergoes sterile filtration (0.2µm) with PBS and subsequently lyophilized.
To reconstitute lyophilized RSPO1, it is recommended to dissolve it in sterile 18MΩ-cm H2O at a concentration of at least 100µg/ml. Further dilutions can be prepared in other aqueous solutions as needed.
Lyophilized RSPO1 demonstrates stability at room temperature for 3 weeks; however, it is advisable to store it desiccated below -18°C. Upon reconstitution, store RSPO1 at 4°C for 2-7 days. For long-term storage, it should be kept below -18°C. It is essential to avoid repeated freeze-thaw cycles to maintain protein integrity.
The purity is determined to be greater than 95.0% as assessed by SDS-PAGE analysis.
The ED50, determined by Luciferase induction in HEK-293 STF cells in the presence of Murine Wnt-3a, is 47.99ng/ml, corresponding to a specific activity of 2.1 x 10^4 units/mg.
R-spondin-1, Cristin-3, mCristin-3, Roof plate-specific spondin-1, Rspo1, RSPO, R-spondin 1, R-spondin, Rspondin, CRISTIN3.
CHO cells.
SRGIKGKRQR RISAEGSQAC AKGCELCSEV NGCLKCSPKL FILLERNDIR QVGVCLPSCP PGYFDARNPD MNKCIKCKIE HCEACFSHNF CTKCKEGLYL HKGRCYPACP EGSSAANGTM ECSSPAQCEM SEWSPWGPCS KKQQLCGFRR GSEERTRRVL HAPVGDHAAC SDTKETRRCT VRRVPCPEGQ KRRKGGQGRR ENANRNLARK ESKEAGAGSR RRKGQQQQQQ QGTVGPLTSA GPA.
Product Science Overview
R-Spondin-1 is a glycosylated protein with a molecular weight of approximately 40 kDa . It acts as a major agonist of the Wnt signaling pathway by regulating the turnover of the LRP6 co-receptor, preventing its internalization by DKK-1 . This regulation enhances Wnt/β-catenin signaling, which is essential for the maintenance and proliferation of stem cells .
Recombinant human R-Spondin-1 (rhRSPO1) is typically produced using mammalian cell expression systems, such as HEK293 cells . The production process involves the synthesis and subcloning of the hRSPO1 coding sequence into a mammalian cell expression vector, followed by stable transfection of the cells . The protein is then purified using chromatographic techniques, ensuring high purity and biological activity .
R-Spondin-1 has several important applications in biomedical research and regenerative medicine. It is used to potentiate Wnt signaling in various organoid culture systems, including intestinal and tumor organoids . Additionally, RSPO1 is crucial for hematopoietic stem cell specification and cancer cell migration and survival . Its mitogenic activity in stem cells makes it a valuable tool in cell therapy and tissue engineering .
