QTRTD1 Human
Queuine TRNA-Ribosyltransferase Domain Containing 1 Human Recombinant
Cat. No.
BT13056
Source
Escherichia Coli.
Synonyms
Queuine TRNA-Ribosyltransferase Domain Containing 1, EC 2.4.2.29, Queuine TRNA-Ribosyltransferase Domain-Containing Protein 1, QTRTD1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
QTRTD1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 438 amino acids (1-415 a.a) and having a molecular mass of 49.1kDa.
QTRTD1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
QTRTD1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
Queuine TRNA-Ribosyltransferase Domain Containing 1, also known as QTRTD1, works in conjunction with QTRT1 to form a functional queuine tRNA-ribosyltransferase enzyme. This enzyme facilitates the replacement of guanine with queuine at the wobble position of tRNAs possessing GUN anticodons (specifically, tRNA-Asp, -Asn, -His, and -Tyr), leading to the formation of the hypermodified nucleoside queuosine.
Description
Recombinant human QTRTD1, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It comprises 438 amino acids (with amino acids 1 to 415 included) and has a molecular weight of 49.1 kDa. A 23 amino acid His-tag is fused to the N-terminus of the protein. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The QTRTD1 protein solution is provided at a concentration of 0.25 mg/ml. The solution is buffered with phosphate-buffered saline at pH 7.4 and supplemented with 30% glycerol, 2 mM DTT, 1 mM EDTA, and 0.1 mM PMSF.
Stability
For short-term storage (2-4 weeks), the product should be kept at 4°C. For longer storage, it is recommended to freeze the product at -20°C. To ensure stability during long-term storage, adding a carrier protein such as HSA or BSA to a final concentration of 0.1% is advised. Repeated freezing and thawing of the product should be avoided.
Purity
The purity of the protein is greater than 95.0%, as determined by SDS-PAGE analysis.
Synonyms
Queuine TRNA-Ribosyltransferase Domain Containing 1, EC 2.4.2.29, Queuine TRNA-Ribosyltransferase Domain-Containing Protein 1, QTRTD1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMKLSLTK VVNGCRLGKI KNLGKTGDHT MDIPGCLLYT KTGSAPHLTH HTLHNIHGVP AMAQLTLSSL AEHHEVLTEY KEGVGKFIGM PESLLYCSLH DPVSPCPAGY VTNKSVSVWS VAGRVEMTVS KFMAIQKALQ PDWFQCLSDG EVSCKEATSI KRVRKSVDRS LLFLDNCLRL QEESEVLQKS VIIGVIEGGD VMEERLRSAR ETAKRPVGGF LLDGFQGNPT TLEARLRLLS SVTAELPEDK PRLISGVSRP DEVLECIERG VDLFESFFPY QVTERGCALT FSFDYQPNPE ETLLQQNGTQ EEIKCMDQIK KIETTGCNQE ITSFEINLKE KKYQEDFNPL VRGCSCYCCK NHTRAYIHHL LVTNELLAGV LLMMHNFEHY FGFFHYIREA LKSDKLAQLK ELIHRQAS.
Product Science Overview
Function and Importance
The primary function of QTRT1 is to catalyze the base-exchange of a guanine residue with queuine at position 34 (the anticodon wobble position) in tRNAs that code for asparagine, aspartic acid, histidine, and tyrosine . This modification is essential for the proper functioning of tRNAs, as it ensures the accuracy and fidelity of protein synthesis by maintaining the correct folding of nascent proteins .
Biological Pathways
Genetic Information
Clinical Significance
Research and Applications
Research into QTRT1 has revealed its importance in maintaining cellular homeostasis and its potential as a therapeutic target. The enzyme’s activity is influenced by the gut microbiome, as the microbiome product queuine is required for its function . This connection highlights the interplay between human genetics and microbiome health.
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