QKI Human
QKI Human Recombinant
Cat. No.
BT10276
Source
Escherichia Coli.
Synonyms
Hqk, hqkI, QK, QK1, QK3, Protein quaking, Quaking
Homolog, KH Domain RNA Binding, RNA Binding Protein HQK, HKQ, KH
Domain Containing, RNA Binding.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
QKI Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 364 amino acids (1-341 a.a) and having a molecular mass of 40.1kDa.
QKI is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
QKI is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
KH Domain RNA Binding, also known as QKI, is an RNA-binding protein that regulates pre-mRNA splicing, mRNA export from the nucleus, protein translation, and mRNA stability. QKI plays a role in myelinization and oligodendrocyte differentiation and may be involved in schizophrenia. Defects or deletions in QKI are linked to astrocytic tumors and may contribute to schizophrenia. QKI is associated with diseases such as 6q terminal deletion syndrome and schizophrenia.
Description
Recombinant human QKI protein, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 364 amino acids (residues 1-341) and possessing a molecular weight of 40.1 kDa. The protein includes a 23 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The QKI protein solution is provided at a concentration of 0.25 mg/ml and is formulated in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.2 M NaCl, 50% glycerol, and 2 mM DTT.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to store the protein at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of the protein is determined to be greater than 85.0% as assessed by SDS-PAGE.
Synonyms
Hqk, hqkI, QK, QK1, QK3, Protein quaking, Quaking
Homolog, KH Domain RNA Binding, RNA Binding Protein HQK, HKQ, KH
Domain Containing, RNA Binding.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMVGEMET KEKPKPTPDY LMQLMNDKKL MSSLPNFCGI FNHLERLLDE EISRVRKDMY NDTLNGSTEK RSAELPDAVG PIVQLQEKLY VPVKEYPDFN FVGRILGPRG LTAKQLEAET GCKIMVRGKG SMRDKKKEEQ NRGKPNWEHL NEDLHVLITV EDAQNRAEIK LKRAVEEVKK LLVPAAEGED SLKKMQLMEL AILNGTYRDA NIKSPALAFS LAATAQAAPR IITGPAPVLP PAALRTPTPA GPTIMPLIRQ IQTAVMPNGT PHPTAAIVPP GPEAGLIYTP YEYPYTLAPA TSILEYPIEP SGVLGAVATK VRRHDMRVHP YQRIVTADRA ATGN.
Product Science Overview
Introduction
QKI, also known as Quaking, is an RNA-binding protein that plays a crucial role in various biological processes, including pre-mRNA splicing, mRNA export from the nucleus, protein translation, and mRNA stability . This protein is part of the signal transduction and activation of RNA (STAR) family and belongs to the heterogeneous nuclear ribonucleoprotein K (hnRNP K) homology domain protein family .
Structure and Isoforms
QKI is located on human chromosome 6 and mouse chromosome 17 . It contains an RNA-binding motif in the KH domain, flanked by two QUA domains (QUA1 and QUA2) . There are three major alternatively spliced isoforms of QKI: QKI-5, QKI-6, and QKI-7 . These isoforms share a common RNA-binding property but differ in their carboxy-terminal domains, which allows them to regulate pre-mRNA splicing, transportation, or stability in a cell type-specific manner .
Biological Functions
QKI is involved in several critical biological functions:
- Pre-mRNA Splicing: QKI regulates alternative splicing, a key mechanism for increasing proteomic diversity and modulating gene expression . For example, QKI-5 inhibits the proliferation and transformation of lung cancer cells by regulating the alternative splicing of NUMB, a protein involved in the Notch signaling pathway .
- Myelinization and Oligodendrocyte Differentiation: QKI plays a significant role in the myelination process and the differentiation of oligodendrocytes, which are essential for the proper functioning of the nervous system .
- Cardiovascular Development: Recent studies have shown that QKI is crucial for cardiovascular development and function . It regulates the differentiation of monocytes to macrophages, bone metabolism, and cancer progression .
Clinical Implications
QKI has been implicated in various diseases and conditions:
- Cancer: QKI is frequently down-regulated in lung cancer, and its down-regulation is significantly associated with a poorer prognosis . It acts as a tumor suppressor by inhibiting cancer cell proliferation and preventing the inappropriate activation of the Notch signaling pathway .
- Neurological Disorders: QKI’s role in myelination and oligodendrocyte differentiation suggests its involvement in neurological disorders such as schizophrenia .
- Cardiovascular Diseases: Given its role in cardiovascular development, QKI may be linked to various cardiovascular pathologies .
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