Influenza-B Qingdao
Influenza-B Virus Qingdao/102/91
Cat. No.
BT27773
Source
Synonyms
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined byAnalysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
Allantoic fluid of 10 days old embryonated eggs, inoculated with influenza B virus, strain B/Qingdao/102/91. The Influenza B Virus was purified by Ultracentrifugation with 10-40 % sucrose gradient.
Product Specs
Introduction
Influenza B virus, a member of the Orthomyxoviridae family, exclusively infects humans and seals. Unlike Influenza A virus, its mutation is limited to genetic drift and reassortment. Influenza B virus evolves at a slower rate compared to Influenza A and a faster rate compared to Influenza C. While Influenza B virus mutates at a rate 2-3 times slower than type A, it still undergoes sufficient mutations to prevent long-lasting immunity. This enveloped virus has a virion structure consisting of a matrix protein, envelope, nucleoprotein complex, nucleocapsid, and polymerase complex. Its morphology can be either spherical or filamentous. The approximately 500 surface projections are composed of hemagglutinin and neuraminidase. The Influenza B virus genome comprises 14,648 nucleotides, organized into eight linear, negative-sense, single-stranded RNA segments. Each segment is enclosed within a separate nucleocapsid, all of which are surrounded by a single envelope.
Description
This product consists of allantoic fluid derived from 10-day-old embryonated eggs inoculated with the B/Qingdao/102/91 strain of Influenza B virus. The virus was purified using ultracentrifugation with a 10-40% sucrose gradient.
Inactivation
This product has been inactivated with Thimerosal and beta-propiolactone treatment. Standard microbiological/viral safe handling practices and techniques are required when working with this product.
Physical Appearance
A sterile-filtered, colorless solution.
Formulation
The B/Qingdao/102/91 solution is formulated with 0.1M NaCl, 10mM Tris HCl, 1mM EDTA (pH 8), 0.1% sodium azide (NaN3), and 0.005% thimerosal.
Stability
For optimal stability, store B/Qingdao/102/91 below -18°C. While it can remain stable at 4°C for up to 4 weeks, it's important to avoid repeated freeze-thaw cycles.
Purity
The purity of this product is greater than 90%, as determined by SDS-PAGE analysis.
Immunological Activity
Serological studies of influenza B virus, immunogen for antibody production.
Tested with anti-influenza B monoclonal antibodies in ELISA.
Tested with anti-influenza B monoclonal antibodies in ELISA.
Product Science Overview
Introduction
Virus Structure and Genome
The Influenza B virus is an enveloped virus with a segmented, negative-sense, single-stranded RNA genome. The genome consists of eight segments, each encapsidated in a separate nucleocapsid and surrounded by an envelope . The virus has surface projections made of hemagglutinin (HA) and neuraminidase (NA), which are crucial for the virus’s ability to infect host cells .
Isolation and Purification
The B/Qingdao/102/91 strain was isolated from allantoic fluid of 10-day-old embryonated eggs inoculated with the virus. The virus was then purified using ultracentrifugation with a 10-40% sucrose gradient . This method ensures high purity, with the virus being greater than 90% pure as determined by SDS-PAGE .
Biological Characteristics
Applications and Research
Storage and Stability
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
