PYCR1 Human

Pyrroline-5-Carboxylate Reductase 1 Human Recombinant
Cat. No.
BT20051
Source
Escherichia Coli.
Synonyms
P5C, PRO3, P5CR 1, Pyrroline-5-Carboxylate Reductase 1 mitochondrial, ARCL2B, PYCR, PIG45, PP222, Proliferation-Inducing Protein 45.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

PYCR1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 339 amino acids (1-319a.a.) and having a molecular mass of 35.5kDa.
PYCR1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
PYCR1, a ubiquitous housekeeping enzyme, catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme also plays a role in generating NADP(+) in specific cell types. PYCR1 forms a homopolymer and is localized in the mitochondria. Mutations in the PYCR1 gene are linked to the autosomal recessive cutis laxa type 2B (ARCL2B).
Description
Recombinant PYCR1 protein, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 339 amino acids (residues 1-319) with a molecular weight of 35.5 kDa. This protein contains a 20 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, sterile filtered solution.
Formulation
The PYCR1 protein solution (0.5 mg/mL) is supplied in 20 mM Tris-HCl buffer (pH 8.5) containing 10% glycerol.
Purity
Purity is determined to be greater than 90% by SDS-PAGE analysis.
Stability
For short-term storage (2-4 weeks), the product should be stored at 4°C. For long-term storage, it is recommended to store the protein at -20°C. Adding a carrier protein such as 0.1% HSA or BSA is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Synonyms
P5C, PRO3, P5CR 1, Pyrroline-5-Carboxylate Reductase 1 mitochondrial, ARCL2B, PYCR, PIG45, PP222, Proliferation-Inducing Protein 45.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MSVGFIGAGQ LAFALAKGFT AAGVLAAHKI MASSPDMDLA TVSALRKMGV KLTPHNKETV QHSDVLFLAV KPHIIPFILD EIGADIEDRH IVVSCAAGVT ISSIEKKLSA FRPAPRVIRC MTNTPVVVRE GATVYATGTH AQVEDGRLME QLLSSVGFCT EVEEDLIDAV TGLSGSGPAY AFTALDALAD GGVKMGLPRR LAVRLGAQAL LGAAKMLLHS EQHPGQLKDN VSSPGGATIH ALHVLESGGF RSLLINAVEA SCIRTRELQS MADQEQVSPA AIKKTILDKV KLDSPAGTAL SPSGHTKLLP RSLAPAGKD

Product Science Overview

Introduction

Pyrroline-5-Carboxylate Reductase 1 (PYCR1) is a mitochondrial enzyme that plays a crucial role in the biosynthesis of proline, an amino acid essential for protein synthesis and cellular metabolism. This enzyme catalyzes the NAD(P)H-dependent conversion of delta1-pyrroline-5-carboxylate (P5C) to proline, which is the final step in proline biosynthesis .

Biological Significance

Proline is not only necessary for maintaining normal physiological functions but also plays a special role in the occurrence of tumors. PYCR1 is overexpressed in various cancers, including breast cancer, lung cancer, prostate cancer, and lymphoma . The enzyme’s overexpression is significantly associated with tumor growth, advanced grades, and poor survival of patients .

Role in Cancer

Recent studies have shown that PYCR1 is involved in the progression of several cancers. Overexpression of PYCR1 is linked to the progression of multiple myeloma, a type of hematological cancer. In multiple myeloma cells, PYCR1 facilitates the conversion of glutamine to proline, contributing to cell survival and drug resistance under hypoxic conditions . Inhibition of PYCR1 has been found to reduce cell viability and proliferation while increasing apoptosis, making it a potential target for cancer therapy .

Enzymatic Function

PYCR1 catalyzes the reduction of delta1-pyrroline-5-carboxylate to proline with the oxidation of NAD(P)H to NAD(P)+ . This enzymatic cycle plays pivotal roles in amino acid metabolism, intracellular redox potential, and mitochondrial integrity . The enzyme is a housekeeping protein, meaning it is essential for the basic maintenance of cellular functions.

Human Recombinant PYCR1

Human recombinant PYCR1 is produced using recombinant DNA technology, which involves inserting the human PYCR1 gene into a suitable expression system, such as bacteria or yeast. This allows for the production of large quantities of the enzyme for research and therapeutic purposes. Recombinant PYCR1 is used in various studies to understand its role in cellular metabolism and its potential as a therapeutic target in cancer treatment.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

PYCRL Human
Pyrroline-5-Carboxylate Reductase Like Human Recombinant
Cat. No.
BT20259
Source
Escherichia Coli.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.