PTTG1 Human

Pituitary Tumor-Transforming Protein 1 Human Recombinant
Cat. No.
BT9954
Source
Escherichia Coli.
Synonyms
Securin, Pituitary tumor-transforming gene 1 protein, hPTTG, Tumor-transforming protein 1, Esp1-associated protein, PTTG1, EAP1, PTTG, TUTR1, MGC126883, MGC138276.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

PTTG1 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 222 amino acids (1- 202 a.a.) and having a molecular mass of 24.1kDa.
The PTTG1 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
PTTG1, also known as Securin, plays a crucial role in regulating sister chromatid separation during cell division. This regulatory protein contributes to chromosome stability, the p53/TP53 pathway, and DNA repair. PTTG1 facilitates the transport of separase (a cysteine protease) to the nucleus and inhibits separase's catalytic activity. The Anaphase Promoting Complex (APC) ubiquitinates PTTG1, leading to its degradation by the Proteasome and the subsequent release of separase. During mitosis, PTTG1 prevents premature chromosome segregation by inhibiting Separase/ESPL1, thereby blocking the proteolysis of the cohesin complex. Importantly, PTTG1's function extends beyond inhibition; it is also required for ESPL1 activation. Ubiquitination of PTTG1 at anaphase onset triggers its destruction and liberates ESPL1. PTTG1 possesses two PXXP motifs essential for its transforming and tumorigenic activities and stimulating basic fibroblast growth factor expression. A D box (destruction box) within PTTG1 is crucial for its APC-mediated degradation. The acidic C-terminal region of Securin can act as a transactivation domain. While primarily cytosolic, PTTG1 partially localizes to the nucleus. Overexpression of PTTG1 is observed in various tumors. It exhibits transforming activity in vitro and tumorigenic activity in vivo. Moreover, PTTG1 negatively regulates TP53's transcriptional activity and associated apoptosis.
Description
Recombinant human PTTG1, fused with a 20 amino acid His tag at its N-terminus, is produced in E. coli. This protein is a single, non-glycosylated polypeptide chain consisting of 222 amino acids (residues 1-202) and has a molecular weight of 24.1 kDa. PTTG1 is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The provided PTTG1 solution has a concentration of 1 mg/ml and is formulated in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.1 M NaCl, 20% glycerol, 1 mM EDTA, and 0.1 mM PMSF.
Stability
For short-term storage (up to 4 weeks), keep the vial refrigerated at 4°C. For extended storage, freeze the solution at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles to prevent protein degradation.
Purity
The purity of PTTG1 is determined to be greater than 90% by SDS-PAGE analysis.
Synonyms
Securin, Pituitary tumor-transforming gene 1 protein, hPTTG, Tumor-transforming protein 1, Esp1-associated protein, PTTG1, EAP1, PTTG, TUTR1, MGC126883, MGC138276.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MATLIYVDKE NGEPGTRVVA KDGLKLGSGP SIKALDGRSQ VSTPRFGKTF DAPPALPKAT RKALGTVNRA TEKSVKTKGP LKQKQPSFSA KKMTEKTVKA KSSVPASDDA YPEIEKFFPF NPLDFESFDL PEEHQIAHLP LSGVPLMILD EERELEKLFQ LGPPSPVKMP SPPWESNLLQ SPSSILSTLD VELPPVCCDI DI.

Product Science Overview

Introduction

Pituitary Tumor-Transforming Protein 1 (PTTG1), also known as securin, is a protein encoded by the PTTG1 gene in humans. It plays a crucial role in cell cycle regulation, particularly in the separation of sister chromatids during mitosis. PTTG1 has garnered significant attention due to its involvement in various cancers and its potential as a therapeutic target.

Discovery and Structure

PTTG1 was first identified in the context of pituitary tumors, where it was found to be overexpressed. The protein consists of 202 amino acids and contains a PXXP motif, which is crucial for its interaction with other proteins. The structure of PTTG1 allows it to bind to and inhibit separase, an enzyme responsible for cleaving cohesin complexes that hold sister chromatids together.

Biological Functions

PTTG1 is a multifunctional protein with several roles in cellular processes:

  • Cell Cycle Regulation: PTTG1 inhibits separase, preventing premature separation of sister chromatids. This ensures proper chromosome segregation during cell division.
  • Transcriptional Regulation: PTTG1 can act as a transcriptional activator, influencing the expression of various genes involved in cell proliferation and survival.
  • DNA Repair: PTTG1 is implicated in DNA repair mechanisms, contributing to genomic stability.
  • Tumorigenesis: Overexpression of PTTG1 is associated with tumorigenesis. It promotes cell proliferation, inhibits apoptosis, and enhances angiogenesis, making it a key player in cancer progression.
Clinical Significance

PTTG1 is overexpressed in various cancers, including pituitary adenomas, breast cancer, gastrointestinal cancers, leukemia, lymphoma, and lung cancer . Its overexpression is often correlated with poor prognosis and increased tumor aggressiveness. Due to its role in tumorigenesis, PTTG1 is considered a potential biomarker for cancer diagnosis and prognosis.

Therapeutic Potential

Given its involvement in cancer, PTTG1 is a promising target for therapeutic interventions. Strategies to inhibit PTTG1 expression or function could potentially suppress tumor growth and improve patient outcomes. Research is ongoing to develop specific inhibitors and immunotherapies targeting PTTG1 .

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