PTPRN Human
Sf9 insect cells.
Receptor-type tyrosine-protein phosphatase-like N, R-PTP-N, Islet cell antigen 512, ICA 512, Islet cell autoantigen 3, PTP IA-2, PTPRN, ICA3, ICA512.
Greater than 95.0% as determined by SDS-PAGE.
Description
Recombinant Human Protein Tyrosine Phosphatase Receptor Type N produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 46kDa.
PTPRN is expressed with a 6xHis tag and purified by proprietary chromatographic techniques.
Product Specs
Protein Tyrosine Phosphatase Receptor Type N (PTPRN), also known as IA-2, is a non-catalytic protein that plays a crucial role in regulating insulin secretion. It is a significant autoantigen in type 1 diabetes. The intracellular C-terminal region of PTPRN contains the majority of the autoantibody binding sites. PTPRN is primarily found in neural, neuroendocrine, and pancreatic islet cells.
Recombinant Human PTPRN, expressed in SF9 insect cells, is a glycosylated polypeptide with an estimated molecular weight of 46kDa. This protein is engineered with a 6xHis tag for purification and is purified using proprietary chromatographic methods.
The PTPRN protein is supplied in a buffer consisting of 50mM Sodium phosphate (pH 8.0) and 20% Glycerol.
For short-term storage (up to 4 weeks), the product can be stored at 4°C. For long-term storage, it is recommended to store the protein at -20°C. Repeated freezing and thawing should be avoided to maintain protein integrity.
The purity of the protein is determined to be greater than 95% as analyzed by SDS-PAGE.
Receptor-type tyrosine-protein phosphatase-like N, R-PTP-N, Islet cell antigen 512, ICA 512, Islet cell autoantigen 3, PTP IA-2, PTPRN, ICA3, ICA512.
Sf9 insect cells.
Product Science Overview
Protein Tyrosine Phosphatase Receptor Type N (PTPRN) is a member of the protein tyrosine phosphatase (PTP) family, which plays a crucial role in cellular signaling. PTPs are enzymes that remove phosphate groups from phosphorylated tyrosine residues on proteins, a process that is essential for regulating various cellular functions such as growth, differentiation, and metabolism.
PTPRN is a receptor-type PTP, meaning it is an integral cell surface protein with both extracellular and intracellular domains. The extracellular domain of PTPRN has sequence homology to cell adhesion molecules, which suggests a role in cell-cell interactions. The intracellular domain contains the phosphatase activity that is responsible for dephosphorylating tyrosine residues on target proteins .
PTPRN is involved in the regulation of secretion pathways in neuroendocrine cells. It has been shown to play a crucial role in the initiation and progression of signaling cascades that regulate cell function . Additionally, PTPRN is associated with various diseases, including insulinoma and type 1 diabetes mellitus . Its role in these diseases highlights its importance in maintaining cellular homeostasis and proper physiological function.
Research on PTPRN has provided significant insights into its function and regulation. Studies have shown that PTPRN is involved in the regulation of insulin secretion and is a target of autoantibodies in type 1 diabetes . This makes it a potential therapeutic target for the treatment of diabetes and other related disorders.
Human recombinant PTPRN is used in research to study its function and to develop potential therapeutic interventions. Recombinant proteins are produced through recombinant DNA technology, which allows for the expression of the protein in a host organism, such as bacteria or yeast. This technology enables researchers to obtain large quantities of the protein for experimental purposes.
