PTPN7 Human

Protein Tyrosine Phosphatase Non Receptor Type-7 Human Recombinant
Cat. No.
BT26073
Source
E.coli.
Synonyms
Protein tyrosine phosphatase non-receptor type 7, HEPTP, LC-PTP, Hematopoietic protein-tyrosine phosphatase, Protein-tyrosine phosphatase LC-PTP, PTPNI, LPTP, BPTP-4, dual specificity phosphatase 1, protein-tyrosine phosphatase non-receptor-type, stress induced, EC 3.1.3.48.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

PTPN7 Human Recombinant produced in E. coli is a single polypeptide chain containing 384 amino acids (1-360) and having a molecular mass of 43.1kDa.
PTPN7 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
PTPN7 is a protein tyrosine phosphatase that specifically dephosphorylates MAPK1 at tyrosine residues. It is involved in the regulation of signal transduction pathways and the development of T and B lymphocytes.
Description
Recombinant human PTPN7 protein was expressed in E. coli and purified to a single polypeptide chain containing 384 amino acids (residues 1-360) with a molecular weight of 43.1 kDa. The protein consists of a 24 amino acid His-tag fused at the N-terminus and was purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile filtered.
Formulation
The PTPN7 protein solution is provided at a concentration of 0.5 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 1 mM DTT, and 20% glycerol.
Stability
For short-term storage (2-4 weeks), store the protein solution at 4°C. For long-term storage, freeze the solution at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for extended storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of the PTPN7 protein is greater than 90% as determined by SDS-PAGE analysis.
Synonyms
Protein tyrosine phosphatase non-receptor type 7, HEPTP, LC-PTP, Hematopoietic protein-tyrosine phosphatase, Protein-tyrosine phosphatase LC-PTP, PTPNI, LPTP, BPTP-4, dual specificity phosphatase 1, protein-tyrosine phosphatase non-receptor-type, stress induced, EC 3.1.3.48.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMVQAHG GRSRAQPLTL SLGAAMTQPP PEKTPAKKHV RLQERRGSNV ALMLDVRSLG AVEPICSVNT PREVTLHFLR TAGHPLTRWA LQRQPPSPKQ LEEEFLKIPS NFVSPEDLDI PGHASKDRYK TILPNPQSRV CLGRAQSQED GDYINANYIR GYDGKEKVYI ATQGPMPNTV SDFWEMVWQE EVSLIVMLTQ LREGKEKCVH YWPTEEETYG PFQIRIQDMK ECPEYTVRQL TIQYQEERRS VKHILFSAWP DHQTPESAGP LLRLVAEVEE SPETAAHPGP IVVHCSAGIG RTGCFIATRI GCQQLKARGE VDILGIVCQL RLDRGGMIQT AEQYQFLHHT LALYAGQLPE EPSP

Product Science Overview

Structure and Function

PTPN7 acts preferentially on tyrosine-phosphorylated MAPK1 (Mitogen-Activated Protein Kinase 1). This protein plays a crucial role in the regulation of T and B-lymphocyte development and signal transduction . The non-catalytic N-terminus of PTPN7 can interact with MAP kinases and suppress their activities, which is essential for the regulation of T cell antigen receptor (TCR) signaling .

Expression and Regulation

PTPN7 is preferentially expressed in a variety of hematopoietic cells and is an early response gene in lymphokine-stimulated cells . The expression of PTPN7 is regulated by various stimuli, including lipopolysaccharide (LPS), which acts as an endotoxin and elicits strong immune responses in animals . Stimulation of RAW 264.7 cells with LPS leads to a transient decrease in the levels of PTPN7 mRNA and protein .

Role in Immune Response

PTPN7 has been shown to act as a negative regulator of pro-inflammatory TNF-α (Tumor Necrosis Factor-alpha) production in macrophages . Overexpression of PTPN7 inhibits LPS-stimulated production of TNF-α, while knock-down of PTPN7 increases TNF-α production . This indicates that PTPN7 plays a critical role in modulating the inflammatory response in macrophages by negatively regulating the extracellular signal-regulated kinase 1/2 (ERK1/2) and p38 pathways .

Clinical Significance

Given its role in regulating immune responses and cell signaling, PTPN7 is of significant interest in the context of diseases such as juvenile myelomonocytic leukemia . Understanding the function and regulation of PTPN7 can provide insights into potential therapeutic targets for treating immune-related disorders and certain types of cancer.

Recombinant PTPN7

Recombinant human PTPN7 is produced in E. coli and is a single polypeptide chain containing 384 amino acids with a molecular mass of 43.1 kDa . This recombinant protein is used in various research applications to study its function and role in cellular processes.

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