PTPN6 Human
Protein Tyrosine Phosphatase Non Receptor Type-6 Human Recombinant
Cat. No.
BT26002
Source
Escherichia Coli.
Synonyms
Tyrosine-protein phosphatase non-receptor type 6, EC 3.1.3.48, Protein-tyrosine phosphatase 1C, PTP-1C, Hematopoietic cell protein-tyrosine phosphatase, SH-PTP1, Protein-tyrosine phosphatase SHP-1, PTPN6, HCP, HCPH, SHP1, HPTP1C, SHP-1L.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY ESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
PTPN6 Human Recombinant produced in E.Coli is a single,non-glycosylated polypeptide chain containing 300 amino acids and having a molecular mass of 34.3 kDa. The protein coding region of the catalytic domain of PTPN6 (amino acids 243-541). The catalytic domain of PTPN6 was overexpressed as insoluble protein aggregates (inclusion bodies). The recombinant PTPN6 protein was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer. Additional amino acid(Met) is attached at N-terminus.
Product Specs
Introduction
Protein tyrosine phosphatase non-receptor type 6 (PTPN6), also known as SHP-1, is a member of the protein tyrosine phosphatase (PTP) family. PTPs are signaling molecules that regulate various cellular processes, including cell growth, differentiation, cell cycle progression, and oncogenic transformation. PTPN6 contains two tandem Src homology 2 (SH2) domains at its N-terminus, which function as protein phospho-tyrosine binding domains and mediate interactions with its substrates. Primarily expressed in hematopoietic cells, PTPN6 plays a crucial role in regulating multiple signaling pathways in these cells. It interacts with and dephosphorylates a wide range of phosphoproteins involved in hematopoietic cell signaling. Several alternatively spliced isoforms of PTPN6 have been identified.
Description
Recombinant human PTPN6, expressed in E. coli, is a non-glycosylated polypeptide chain comprising 300 amino acids. It encompasses the catalytic domain of PTPN6 (amino acids 243-541) and has a molecular weight of 34.3 kDa. The catalytic domain was overexpressed in E. coli as insoluble protein aggregates (inclusion bodies). After refolding the isolated inclusion bodies in a redox buffer, recombinant PTPN6 was purified using FPLC gel-filtration chromatography. An additional methionine residue is present at the N-terminus.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The protein is supplied in a solution containing 25mM Tris-HCl (pH 7.5), 2mM b-mercaptoethanol, 1mM EDTA, 1mM DTT, and 20% glycerol.
Stability
For short-term storage (up to 4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to store the protein at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is advisable for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity of the protein is greater than 95% as determined by SDS-PAGE analysis.
Specific Activity
The specific activity of PTPN6 is greater than 5,000 units per milligram (U/mg).
Unit Definition
One unit of PTPN6 activity is defined as the amount of enzyme required to hydrolyze 1 nanomole of p-nitrophenylphosphate per minute at a pH of 7.5 and a temperature of 37°C, using a substrate concentration of 10mM.
Synonyms
Tyrosine-protein phosphatase non-receptor type 6, EC 3.1.3.48, Protein-tyrosine phosphatase 1C, PTP-1C, Hematopoietic cell protein-tyrosine phosphatase, SH-PTP1, Protein-tyrosine phosphatase SHP-1, PTPN6, HCP, HCPH, SHP1, HPTP1C, SHP-1L.
Source
Escherichia Coli.
Amino Acid Sequence
MGFWEEFES LQKQEVKNLH QRLEGQRPEN KGKNRYKNIL PFDHSRVILQ GRDSNIPGSD YINANYIKNQ LLGPDENAKT YIASQGCLEA TVNDFWQMAW QENSRVIVMT TREVEKGRNK CVPYWPEVGM QRAYGPYSVT NCGEHDTTEY KLRTLQVSPL DNGDLIREIW HYQYLSWPDH GVPSEPGGVL SFLDQINQRQ ESLPHAGPII VHCSAGIGRT GTIIVIDMLM ENISTKGLDCDIDIQKTIQM VRAQRSGMVQ TEAQYKFIYV AIAQFIETTK KKLEVLQSQK GQESEYGNITY.
Product Science Overview
Structure and Function
The PTPN6 protein contains two tandem Src homology 2 (SH2) domains at its N-terminal, which act as protein phospho-tyrosine binding domains. These domains mediate the interaction of PTPN6 with its substrates . The primary function of PTPN6 is to dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling .
Expression and Regulation
PTPN6 is primarily expressed in hematopoietic cells, where it functions as an important regulator of multiple signaling pathways . The expression of SHP-1 is regulated by two promoters: P-1, which is active in epithelial cells, and P-2, which is active in hematopoietic cells . Interestingly, the expression levels of SHP-1 vary between cell types and can change in response to cancer, with increased levels in epithelial cells and decreased levels in hematopoietic cells .
Clinical Significance
Research and Applications
Recombinant human PTPN6 is used extensively in research to study its role in cell signaling and its potential as a therapeutic target. The availability of human recombinant PTPN6 allows for detailed biochemical studies and the development of inhibitors that could modulate its activity in disease contexts .
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