PTPN4 Human

Protein Tyrosine Phosphatase Non Receptor Type-4 Human Recombinant
Cat. No.
BT25901
Source
E.coli.
Synonyms
Protein Tyrosine Phosphatase, Non-Receptor Type 4 (Megakaryocyte), Protein-Tyrosine Phosphatase MEG1, PTPase-MEG1, EC 3.1.3.48, MEG, Tyrosine-Protein Phosphatase Non-Receptor Type 4, Megakaryocyte Protein-Tyrosine Phosphatase, Protein Tyrosine Phosphatase MEG1, Megakaryocyte Phosphatase, PTPMEG1, PTPMEG, Tyrosine-protein phosphatase non-receptor type 4, Protein-tyrosine phosphatase MEG1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

PTPN4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 280 amino acids (655-913 a.a) and having a molecular mass of 32kDa.
PTPN4 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Protein Tyrosine Phosphatase Non Receptor Type-4, also known as PTPN4, is a member of the protein tyrosine phosphatase (PTP) family. PTPs are signaling molecules that play crucial roles in regulating various cellular processes, including cell growth, differentiation, cell cycle progression, and oncogenic transformation. PTPN4 is a widely expressed non-receptor protein tyrosine phosphatase. It interacts with glutamate receptors, such as GluRδ2 and GluRε, and influences glutamate receptor signaling and/or regulates their activity via tyrosine dephosphorylation.
Description
Recombinant human PTPN4 protein, expressed in E. coli, is a non-glycosylated polypeptide chain consisting of 280 amino acids (residues 655-913). It has a molecular weight of 32 kDa. The protein includes a 21 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
The PTPN4 protein solution has a concentration of 1 mg/ml and is supplied in a buffer containing 20 mM Tris (pH 8.0) and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is advisable for long-term storage. Repeated freezing and thawing of the product should be avoided.
Purity
The purity of the PTPN4 protein is greater than 85%, as determined by SDS-PAGE analysis.
Synonyms
Protein Tyrosine Phosphatase, Non-Receptor Type 4 (Megakaryocyte), Protein-Tyrosine Phosphatase MEG1, PTPase-MEG1, EC 3.1.3.48, MEG, Tyrosine-Protein Phosphatase Non-Receptor Type 4, Megakaryocyte Protein-Tyrosine Phosphatase, Protein Tyrosine Phosphatase MEG1, Megakaryocyte Phosphatase, PTPMEG1, PTPMEG, Tyrosine-protein phosphatase non-receptor type 4, Protein-tyrosine phosphatase MEG1.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MVLTQFDQLY RKKPGMTMSC AKLPQNISKN RYRDISPYDA TRVILKGNED YINANYINME IPSSSIINQY IACQGPLPHT CTDFWQMTWE QGSSMVVMLT TQVERGRVKC HQYWPEPTGS SSYGCYQVTC HSEEGNTAYI FRKMTLFNQE KNESRPLTQI QYIAWPDHGV PDDSSDFLDF VCHVRNKRAG KEEPVVVHCS AGIGRTGVLI TMETAMCLIE CNQPVYPLDI VRTMRDQRAM MIQTPSQYRF VCEAILKVYE.

Product Science Overview

Introduction

Protein Tyrosine Phosphatases (PTPs) are a group of enzymes that play a crucial role in regulating cellular processes by dephosphorylating tyrosine residues on proteins. Among these, Protein Tyrosine Phosphatase Non-Receptor Type-4 (PTPN4) is a significant member that has garnered attention due to its involvement in various cellular functions and disease mechanisms.

Structure and Function

PTPN4 is characterized by its structure, which includes a C-terminal PTP domain and an N-terminal domain homologous to the band 4.1 superfamily of cytoskeletal-associated proteins . This structural composition allows PTPN4 to interact with various cellular components and participate in signaling pathways.

The primary function of PTPN4 is to dephosphorylate receptor protein tyrosine kinases such as INSR, EGFR, CSF1R, and PDGFR, as well as non-receptor protein tyrosine kinases like JAK1, JAK2, JAK3, and Src family kinases . This dephosphorylation activity is crucial for regulating cell growth, differentiation, mitotic cycle, and oncogenic transformation.

Role in Cellular Processes

PTPN4 is involved in several cellular processes, including:

  • Cell Growth and Differentiation: By regulating the phosphorylation state of key signaling molecules, PTPN4 influences cell growth and differentiation.
  • Mitotic Cycle: PTPN4 plays a role in the mitotic cycle, ensuring proper cell division and proliferation.
  • Oncogenic Transformation: Dysregulation of PTPN4 activity can lead to uncontrolled cell growth and cancer development.
Clinical Significance

The expression patterns of PTPN4 and other PTPN family members have been studied extensively in various cancers, including digestive tract cancers . Alterations in PTPN4 expression are associated with the incidence, prognosis, and pathological grade of these cancers. For instance, high expression levels of PTPN4 have been observed in esophageal and gastric cancer cell lines .

Research and Therapeutic Potential

Research on PTPN4 continues to uncover its potential as a therapeutic target. By understanding the molecular mechanisms regulated by PTPN4, scientists aim to develop targeted therapies for diseases associated with its dysregulation. The recombinant form of human PTPN4 is used in research to study its function and to screen for potential inhibitors that could be used in cancer treatment.

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