PTPN11 Human
Protein Tyrosine Phosphatase, Non-Receptor Type 11, Protein-Tyrosine Phosphatase 1D, Protein-Tyrosine Phosphatase 2C, EC 3.1.3.48, SH-PTP2, SH-PTP3, PTP-1D, PTP-2C, PTP2C, SHP2, Tyrosine-Protein Phosphatase Non-Receptor Type 11, Noonan Syndrome 1, METCDS, SHPTP2, BPTP3, SHP-2, JMML, NS1, CFC, PTPN11.
Greater than 90.0% as determined by SDS-PAGE.
Description
PTPN11 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 469 amino acids (1-460a.a.) and having a molecular mass of 53.9kDa. PTPN11 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Protein Tyrosine Phosphatase, Non-Receptor Type 11, Protein-Tyrosine Phosphatase 1D, Protein-Tyrosine Phosphatase 2C, EC 3.1.3.48, SH-PTP2, SH-PTP3, PTP-1D, PTP-2C, PTP2C, SHP2, Tyrosine-Protein Phosphatase Non-Receptor Type 11, Noonan Syndrome 1, METCDS, SHPTP2, BPTP3, SHP-2, JMML, NS1, CFC, PTPN11.
ADPMTSRRWF HPNITGVEAE NLLLTRGVDG SFLARPSKSN PGDFTLSVRR NGAVTHIKIQ NTGDYYDLYG GEKFATLAEL VQYYMEHHGQ LKEKNGDVIE LKYPLNCADP TSERWFHGHL SGKEAEKLLT EKGKHGSFLV RESQSHPGDF VLSVRTGDDK GESNDGKSKV THVMIRCQEL KYDVGGGERF DSLTDLVEHY KKNPMVETLG TVLQLKQPLN TTRINAAEIE SRVRELSKLA ETTDKVKQGF WEEFETLQQQ ECKLLYSRKE GQRQENKNKN RYKNILPFDH TRVVLHDGDP NEPVSDYINA NIIMPEFETK CNNSKPKKSY IATQGCLQNT VNDFWRMVFQ ENSRVIVMTT KEVERGKSKC VKYWPDEYAL KEYGVMRVRN VKESAAHDYT LRELKLSKVG QGNTERTVWQ YHFRTWPDHG VPSDPGGVLD FLEEVHHKQE SIMDAGPVVV HCRHHHHHH
Product Science Overview
The PTPN11 gene encodes the SHP-2 protein, which contains two tandem Src homology-2 (SH2) domains. These domains function as phospho-tyrosine binding domains and mediate the interaction of SHP-2 with its substrates . The protein is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are crucial for cell functions such as mitogenic activation, metabolic control, transcription regulation, and cell migration .
SHP-2 acts downstream of various receptor and cytoplasmic protein tyrosine kinases, participating in signal transduction from the cell surface to the nucleus . It positively regulates the MAPK signal transduction pathway and dephosphorylates several substrates, including GAB1, ARHGAP35, EGFR, ROCK2, CDC73, and SOX9 . This dephosphorylation activity is essential for the stimulation and activation of the Erk/MAP kinase pathway via signals from tyrosine kinases .
Mutations in the PTPN11 gene are associated with several diseases, including Noonan syndrome and acute myeloid leukemia . During embryonic development, SHP-2 is critical for the development of the heart, blood cells, bones, and other tissues . The PTPN11 gene belongs to a class of genes known as oncogenes, which have the potential to cause normal cells to become cancerous when mutated .
