PTPN11 Human, Active
Sf9, Insect cells.
PTPN11, Tyrosine-protein phosphatase non-receptor type 11, Protein-tyrosine phosphatase 1D, PTP-1D, Proteintyrosine phosphatase 2C, PTP-2C, SH-PTP2, SH-PTP3, BPTP3, CFC, JMML, METCDS, NS1, SHP-2, shp-2, PTP2C, SHPTP2.
Greater than 90.0% as determined by SDS-PAGE.
Description
PTPN11 Human produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 602 amino acids ( 1-593 a.a.) and having a molecular mass of 69.1 kDa.
PTPN11 is expressed with a 9 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
PTPN11, Tyrosine-protein phosphatase non-receptor type 11, Protein-tyrosine phosphatase 1D, PTP-1D, Proteintyrosine phosphatase 2C, PTP-2C, SH-PTP2, SH-PTP3, BPTP3, CFC, JMML, METCDS, NS1, SHP-2, shp-2, PTP2C, SHPTP2.
Sf9, Insect cells.
ADPMTSRRWF HPNITGVEAE NLLLTRGVDG SFLARPSKSN PGDFTLSVRR NGAVTHIKIQ NTGDYYDLYG GEKFATLAEL VQYYMEHHGQ LKEKNGDVIE LKYPLNCADP TSERWFHGHL SGKEAEKLLT EKGKHGSFLV RESQSHPGDF VLSVRTGDDK GESNDGKSKV THVMIRCQEL KYDVGGGERF DSLTDLVEHY KKNPMVETLG TVLQLKQPLN TTRINAAEIE SRVRELSKLA ETTDKVKQGF WEEFETLQQQ ECKLLYSRKE GQRQENKNKN RYKNILPFDH TRVVLHDGDP NEPVSDYINA NIIMPEFETK CNNSKPKKSY IATQGCLQNT VNDFWRMVFQ ENSRVIVMTT KEVERGKSKC VKYWPDEYAL KEYGVMRVRN VKESAAHDYT LRELKLSKVG QGNTERTVWQ YHFRTWPDHG VPSDPGGVLD FLEEVHHKQE SIMDAGPVVV HCSAGIGRTG TFIVIDILID
IIREKGVDCD IDVPKTIQMV RSQRSGMVQT EAQYRFIYMA VQHYIETLQR RIEEEQKSKR KGHEYTNIKY SLADQTSGDQ SPLPPCTPTP PCAEMREDSA RVYENVGLMQ QQKSFRHHHH HH
Product Science Overview
PTPN11 is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration . The enzyme acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in signal transduction from the cell surface to the nucleus .
In its inactive state, the N-terminal SH2 domain binds the PTP domain and blocks access of potential substrates to the active site. Upon binding to target phospho-tyrosyl residues, the N-terminal SH2 domain is released from the PTP domain, catalytically activating the enzyme by relieving this auto-inhibition .
Mutations in the PTPN11 gene are associated with several genetic disorders, including Noonan syndrome and acute myeloid leukemia . Noonan syndrome is characterized by distinctive facial features, short stature, and congenital heart defects. Acute myeloid leukemia is a type of cancer that affects the blood and bone marrow .
Recombinant PTPN11 is used in research to study its role in various signaling pathways and its involvement in diseases. It is responsible for the catalyzation of tyrosine residues dephosphorylation in proteins and takes part in the stimulation and activation of Erk/MAP kinase transduction via signals from tyrosine kinase .
