PSMF1 Human
Description
PSMF1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
The proteasome is a large protease complex composed of 28 subunits arranged in four heptameric rings, forming a cylindrical structure . The core of the proteasome, known as the 20S core, is responsible for the proteolytic activity, while the 19S regulator assists in recognizing and unfolding ubiquitinated proteins for degradation .
PSMF1 functions as an inhibitory subunit of the proteasome. It specifically inhibits the activation of the proteasome by the regulatory proteins PA700 and PA28 . This inhibition is crucial for maintaining the balance of protein degradation within the cell, preventing excessive proteolysis that could be detrimental to cellular function.
The PSMF1 gene is located on chromosome 20p13 and encodes a protein with a molecular mass of approximately 29.8 kDa . The protein consists of a coiled-coil N-terminal domain and a proline-rich C-terminal domain, which is essential for its inhibitory function . The inhibitory activity of PI31 is mediated through its interaction with the 20S proteasome, blocking the assembly of the 26S proteasome from its subcomplexes .
Proteasome inhibitors like PI31 are vital for regulating the proteolytic activity of the proteasome. By inhibiting the proteasome, PI31 helps control the degradation of proteins, ensuring that only damaged or unneeded proteins are targeted for destruction. This regulation is essential for maintaining cellular homeostasis and preventing diseases associated with protein misfolding and aggregation, such as neurodegenerative disorders .
Research has shown that PI31 can inhibit the hydrolysis of protein and peptide substrates by the 20S proteasome, without affecting other proteinases . This specificity makes PI31 a potential target for therapeutic interventions in diseases where proteasome activity is dysregulated. For example, proteasome inhibitors are being explored as treatments for multiple myeloma, a type of cancer characterized by excessive protein degradation .
In conclusion, Proteasome Inhibitor Subunit 1 (Human Recombinant) is a critical component in the regulation of proteasome activity, playing a significant role in maintaining cellular protein homeostasis. Its inhibitory function and potential therapeutic applications make it a valuable subject of ongoing research.
