PSME3 Human

Proteasome Activator Subunit 3 Human Recombinant
Cat. No.
BT7655
Source
E.coli.
Synonyms
Proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki), PA28G, Ki, Ki nuclear autoantigen, PA28-gamma, REG-GAMMA, Activator of multicatalytic protease subunit 3, Proteasome activator 28 subunit gamma, 11S regulator complex subunit gamma, PA28gamma,
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

PSME3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 274 amino acids (1-254 a.a.) and having a molecular mass of 31.7kDa.
PSME3 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
PSME3, a member of the PA28 family, plays a crucial role in the ubiquitin-proteasome system. This system involves the 26S proteasome, a complex structure made up of a 20S core and a 19S regulator, responsible for degrading proteins in the cell. PSME3 specifically activates the trypsin-like activity within the proteasome while inhibiting chymotrypsin-like and postglutamyl-preferring activities. It also interacts with MDM2 and p53, promoting the degradation of p53 and ultimately suppressing cell growth and apoptosis following DNA damage.
Description
Recombinant human PSME3, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 274 amino acids, including a 20-amino acid His-tag at the N-terminus, resulting in a molecular weight of 31.7 kDa. The protein is purified using proprietary chromatographic techniques to ensure high purity.
Physical Appearance
Clear, colorless solution sterilized by filtration.
Formulation
The PSME3 protein is supplied as a 0.5 mg/ml solution in a buffer containing 20mM Tris-HCl (pH 8.0), 200mM NaCl, 2mM DTT, and 40% glycerol.
Stability
For short-term storage (2-4 weeks), the protein can be stored at 4°C. For long-term storage, it is recommended to store the protein at -20°C. Adding a carrier protein such as HSA or BSA (0.1%) is recommended for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity of PSME3 is greater than 90% as determined by SDS-PAGE analysis.
Synonyms
Proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki), PA28G, Ki, Ki nuclear autoantigen, PA28-gamma, REG-GAMMA, Activator of multicatalytic protease subunit 3, Proteasome activator 28 subunit gamma, 11S regulator complex subunit gamma, PA28gamma,
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MASLLKVDQE VKLKVDSFRE RITSEAEDLV ANFFPKKLLE LDSFLKEPIL NIHDLTQIHS DMNLPVPDPI LLTNSHDGLD GPTYKKRRLD ECEEAFQGTK VFVMPNGMLK SNQQLVDIIE KVKPEIRLLI EKCNTVKMWV QLLIPRIEDG NNFGVSIQEE TVAELRTVES EAASYLDQIS RYYITRAKLV SKIAKYPHVE DYRRTVTEID EKEYISLRLI ISELRNQYVT LHDMILKNIE KIKRPRSSNA ETLY

Product Science Overview

Structure and Function

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of two complexes: a 20S core and a 19S regulator . The 20S core is made up of four rings of 28 non-identical subunits, with two rings composed of seven alpha subunits and two rings composed of seven beta subunits . The 19S regulator consists of a base, which contains six ATPase subunits and two non-ATPase subunits, and a lid, which contains up to ten non-ATPase subunits .

PSME3 is a subunit of the 11S regulator, which is an alternate regulator of the proteasome . The 11S regulator, also known as PA28, replaces the 19S regulator in the immunoproteasome, a modified form of the proteasome that processes class I MHC peptides . The gamma subunit of the 11S regulator, encoded by the PSME3 gene, forms a homohexameric ring composed of six gamma subunits .

Biological Significance

PSME3 plays a significant role in various cellular processes, including the regulation of the cell cycle, apoptosis, and the immune response . It is involved in the degradation of proteins that are ubiquitinated, a process that tags proteins for degradation by the proteasome . The immunoproteasome, which includes the 11S regulator, is essential for the processing of class I MHC peptides, which are crucial for the immune response .

Clinical Relevance

Mutations or dysregulation of the PSME3 gene have been associated with several diseases, including systemic lupus erythematosus and ovarian cancer . The protein’s involvement in the degradation of regulatory proteins makes it a potential target for therapeutic interventions in these diseases .

Research and Applications

Recombinant PSME3 is used in various research applications to study its structure, function, and role in disease processes . Understanding the mechanisms by which PSME3 regulates the proteasome and its involvement in cellular processes can provide insights into potential therapeutic targets for diseases associated with proteasome dysfunction .

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THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
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