PSMD5 Human

Proteasome 26S Subunit, Non-ATPase 5 Human Recombinant
Cat. No.
BT7356
Source
E.coli.
Synonyms
PSMD5, Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 526S Protease Subunit S5 Basic,26S Proteasome Subunit S5B,S5B,26S Proteasome Non-ATPase Regulatory Subunit 5,KIAA0072.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

PSMD5 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 529 amino acids (1-504 a.a) and having a molecular mass of 58.9kDa.
PSMD5 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Proteasome 26S Subunit, Non-ATPase 5, also known as PSMD5, is a component of the proteasome subunit S5B/HSM3 family. This protein plays a role in the 26S proteasome, a complex responsible for degrading ubiquitinated proteins within eukaryotic cells. PSMD5 functions as a chaperone protein, contributing to the assembly of the 26s proteasome, specifically the base subcomplex of the PA700/19S regulatory complex. It is characterized by numerous dileucine repeats, which are believed to be involved in the trafficking of various transmembrane proteins.
Description
Recombinant human PSMD5, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 529 amino acids (specifically, residues 1-504). With a molecular weight of 58.9 kDa, this protein is fused to a 25 amino acid His-tag at its N-terminus. Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The PSMD5 protein solution is provided at a concentration of 1 mg/ml and is prepared in a buffer consisting of Phosphate Buffered Saline (pH 7.4) with 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. To maintain product integrity, avoid repeated cycles of freezing and thawing.
Purity
The purity of the PSMD5 protein is greater than 90.0%, as determined by SDS-PAGE analysis.
Synonyms
PSMD5, Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 526S Protease Subunit S5 Basic,26S Proteasome Subunit S5B,S5B,26S Proteasome Non-ATPase Regulatory Subunit 5,KIAA0072.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSEFMAAQA LALLREVARL EAPLEELRAL HSVLQAVPLN ELRQQAAELR LGPLFSLLNE NHREKTTLCV SILERLLQAM EPVHVARNLR VDLQRGLIHP DDSVKILTLS QIGRIVENSD AVTEILNNAE LLKQIVYCIG GENLSVAKAA IKSLSRISLT QAGLEALFES NLLDDLKSVM KTNDIVRYRV YELIIEISSV SPESLNYCTT SGLVTQLLRE LTGEDVLVRA TCIEMVTSLA YTHHGRQYLA QEGVIDQISN IIVGADSDPF SSFYLPGFVK FFGNLAVMDS PQQICERYPI FVEKVFEMIE SQDPTMIGVA VDTVGILGSN VEGKQVLQKT GTRFERLLMR IGHQSKNAPV ELKIRCLDAI SSLLYLPPEQ QTDDLLRMTE SWFSSLSRDP LELFRGISSQ PFPELHCAAL KVFTAIANQP WAQKLMFNSP GFVEYVVDRS VEHDKASKDA KYELVKALAN SKTIAEIFGN PNYLRLRTYL SEGPYYVKPV STTAVEGAE.

Product Science Overview

Introduction

Proteasome 26S Subunit, Non-ATPase 5 (PSMD5) is a component of the 26S proteasome, a complex and essential protease machinery in eukaryotic cells. The 26S proteasome is responsible for the ATP-dependent degradation of ubiquitinated proteins, playing a crucial role in maintaining cellular homeostasis by removing misfolded, damaged, or unneeded proteins .

Structure and Function

The 26S proteasome is a large, multi-subunit complex composed of a 20S core particle and a 19S regulatory particle. PSMD5 is one of the non-ATPase subunits of the 19S regulatory particle. This subunit is involved in recognizing and binding ubiquitinated substrates, facilitating their translocation into the 20S core for degradation .

Biological Significance

PSMD5, along with other subunits of the 26S proteasome, is essential for various cellular processes, including cell cycle regulation, signal transduction, and stress responses. The degradation of ubiquitinated proteins by the proteasome is a highly regulated process, ensuring that only proteins tagged for destruction are targeted, thus maintaining protein homeostasis .

Research and Applications

Recombinant human PSMD5 is used in research to study the structure and function of the 26S proteasome. Advances in cryogenic electron microscopy (cryo-EM) have provided detailed insights into the dynamic organization and functional mechanisms of the proteasome, including the role of PSMD5 in substrate recognition and processing .

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