PSMD11 Human

Proteasome 26S Subunit, Non-ATPase 11 Human Recombinant
Cat. No.
BT7176
Source
Escherichia Coli.
Synonyms
S9, Rpn6, p44.5, MGC26S proteasome regulatory subunit S9, 26S proteasome regulatory subunit p44.5, PSMD11.3844, 26S proteasome non-ATPase regulatory subunit 11, 26S proteasome regulatory subunit RPN6.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

PSMD11 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 442 amino acids (1-422a.a) and having a molecular mass of 49.6kDa. PSMD11 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
The 26S proteasome, a crucial multicatalytic proteinase complex, comprises a 20S core and a 19S regulator. PSMD11, a non-ATPase subunit, is located within the 19S regulator. The 20S core consists of four rings formed by 28 distinct subunits, with two rings each of 7 alpha and 7 beta subunits. In contrast, the 19S regulator includes a base with six ATPase and two non-ATPase subunits, and a lid with up to ten non-ATPase subunits. Abundant throughout eukaryotic cells, proteasomes participate in the ATP/ubiquitin-dependent degradation of peptides through a non-lysosomal pathway. Notably, the immunoproteasome, a modified proteasome, plays a vital role in processing class I MHC peptides.
Description
Recombinant human PSMD11, expressed in E.coli, is a single, non-glycosylated polypeptide chain. It consists of 442 amino acids (1-422a.a), resulting in a molecular weight of 49.6kDa. The protein includes a 20 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A clear solution that has been sterilized by filtration.
Formulation
The PSMD11 solution is provided at a concentration of 1mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0) and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the solution should be kept at 4°C. For extended storage, it's recommended to freeze the solution at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Repeated freezing and thawing of the solution should be avoided.
Purity
The purity of PSMD11 is greater than 85% as determined by SDS-PAGE analysis.
Synonyms
S9, Rpn6, p44.5, MGC26S proteasome regulatory subunit S9, 26S proteasome regulatory subunit p44.5, PSMD11.3844, 26S proteasome non-ATPase regulatory subunit 11, 26S proteasome regulatory subunit RPN6.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MAAAAVVEFQ RAQSLLSTDR EASIDILHSI VKRDIQENDE EAVQVKEQSI LELGSLLAKT GQAAELGGLL KYVRPFLNSI SKAKAARLVR SLLDLFLDME AATGQEVELC LECIEWAKSE KRTFLRQALE ARLVSLYFDT KRYQEALHLG SQLLRELKKM DDKALLVEVQ LLESKTYHAL SNLPKARAAL TSARTTANAI YCPPKLQATL DMQSGIIHAA EEKDWKTAYS YFYEAFEGYD SIDSPKAITS LKYMLLCKIM LNTPEDVQAL VSGKLALRYA GRQTEALKCV AQASKNRSLA DFEKALTDYR AELRDDPIIS THLAKLYDNL LEQNLIRVIE PFSRVQIEHI SSLIKLSKAD VERKLSQMIL DKKFHGILDQ GEGVLIIFDE PPVDKTYEAA LETIQNMSKV VDSLYNKAKK LT.

Product Science Overview

Introduction

The Proteasome 26S Subunit, Non-ATPase 11 (PSMD11), also known as RPN6, is a crucial component of the 26S proteasome complex in humans. This subunit plays a significant role in the ATP-dependent degradation of ubiquitinated proteins, which is essential for maintaining cellular homeostasis by removing misfolded, damaged, or unneeded proteins .

Structure and Function

The 26S proteasome is a large, multi-catalytic proteinase complex composed of two main subcomplexes: the 20S core particle and the 19S regulatory particle. The 20S core is made up of four rings of 28 non-identical subunits, while the 19S regulatory particle consists of a base and a lid. PSMD11 is a non-ATPase subunit located in the lid of the 19S regulatory particle .

PSMD11 is involved in the assembly and stability of the 26S proteasome. It is particularly important in embryonic stem cells, where its high expression promotes enhanced proteasome activity, which is crucial for the rapid turnover of proteins during cell differentiation and development .

Biological Significance

The 26S proteasome, including PSMD11, is vital for numerous cellular processes, such as:

  • Cell cycle progression: By degrading cyclins and other regulatory proteins, the proteasome ensures proper cell cycle transitions.
  • Apoptosis: The proteasome regulates the levels of pro- and anti-apoptotic proteins, thereby controlling programmed cell death.
  • DNA damage repair: By removing damaged proteins and regulating the levels of repair enzymes, the proteasome plays a role in maintaining genomic integrity .
Clinical Relevance

Mutations or dysregulation of PSMD11 and other proteasome subunits have been associated with various diseases, including cancer and neurodegenerative disorders. For instance, altered proteasome activity can lead to the accumulation of damaged proteins, contributing to the pathogenesis of diseases like Alzheimer’s and Parkinson’s .

Research and Therapeutic Potential

Given its central role in protein homeostasis, the proteasome, including PSMD11, is a target for therapeutic interventions. Proteasome inhibitors, such as bortezomib, are already used in the treatment of multiple myeloma and other cancers. Understanding the specific functions and regulation of PSMD11 could lead to the development of more targeted therapies for a range of diseases .

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