PSMD10 Antibody
Gankyrin, Mouse Anti Human
Cat. No.
BT24542
Source
Synonyms
26S proteasome non-ATPase regulatory subunit 10, 26S proteasome regulatory subunit p28, Gankyrin, PSMD10, p28, dJ889N15.2.
Appearance
Sterile filtered colorless solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Product Specs
Introduction
Gankyrin, also known as proteasome 26S subunit, is an oncoprotein frequently overexpressed in hepatocellular carcinomas. It functions as a regulatory subunit of the 26S proteasome, which plays a crucial role in the ATP-dependent degradation of ubiquitinated proteins. Gankyrin interacts with the S6 ATPase within the 19S regulatory particle of the 26S proteasome. This protein is involved in regulating retinoblastoma protein phosphorylation by CDK4 and enhancing p53 ubiquitination by MDM2. Structurally similar to I kappa Bs, Gankyrin comprises seven ankyrin repeats. Gankyrin has been implicated in the progression of esophageal squamous cell carcinoma and is believed to play an oncogenic role, particularly in the early stages of human hepatocarcinogenesis. It binds to NF-kappaB, suppressing its transcriptional activity by modulating acetylation via SIRT1. Structural comparisons between Gankyrin and p16(INK4A) have revealed several gankyrin residues potentially significant for CDK4 binding.
Physical Appearance
A clear and sterile solution without any color.
Formulation
The antibody solution is provided at a concentration of 1mg/ml in a buffer consisting of PBS at pH 7.4, 10% glycerol, and 0.02% sodium azide.
Storage Procedures
For storage up to 1 month, maintain the antibody at 4°C. For prolonged storage, store at -20°C. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
The antibody remains stable for 12 months when stored at -20°C and for 1 month at 4°C.
Applications
PSMD10 antibody has been validated for use in ELISA, Western blot analysis, Flow cytometry, and ICC/IF to ensure its specificity and reactivity. However, optimal working dilutions should be determined empirically for each application to achieve the best results.
Synonyms
26S proteasome non-ATPase regulatory subunit 10, 26S proteasome regulatory subunit p28, Gankyrin, PSMD10, p28, dJ889N15.2.
Purification Method
PSMD10 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT1F4AT.
Immunogen
Anti-human PSMD10 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PSMD10 protein 1-226 amino acids purified from E.coli.
Ig Subclass
Mouse IgG1 heavy chain and k light chain.
Product Science Overview
Gankyrin: An Overview
Gankyrin, also known as PSMD10 (26S proteasome non-ATPase regulatory subunit 10), is a protein that plays a crucial role in the regulation of the proteasome, a complex responsible for degrading unneeded or damaged proteins by proteolysis, a chemical reaction that breaks peptide bonds . Gankyrin is characterized by its ankyrin repeat domains, which are known for mediating protein-protein interactions .
Biological Significance
Gankyrin is highly expressed in various cancers, including hepatocellular carcinoma, where it contributes to tumorigenesis by promoting cell proliferation and inhibiting apoptosis . It interacts with several key regulatory proteins, such as the retinoblastoma protein (Rb) and MDM2, an E3 ubiquitin-protein ligase, thereby influencing cell cycle progression and survival .
Mouse Anti-Human Gankyrin Antibodies
Applications in Research
- Western Blotting (WB): Mouse anti-human Gankyrin antibodies are used to detect Gankyrin protein levels in cell and tissue lysates. This application helps in understanding the expression patterns of Gankyrin in different biological contexts .
- Flow Cytometry (Flow Cyt): These antibodies are also employed in flow cytometry to analyze the presence and quantity of Gankyrin in various cell populations .
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