PSIP1 Human

PC4 And SFRS1 Interacting Protein 1 Human Recombinant
Cat. No.
BT9596
Source
Sf9 insect cells.
Synonyms
PC4 and SFRS1 Interacting Protein 1, Lens Epithelium-Derived Growth Factor, Transcriptional Coactivator P75/P52, Dense Fine Speckles 70 KDa Protein, CLL-Associated Antigen KW-7, DFS 70, PSIP2, DFS70, LEDGF, PC4 And SFRS1 Interacting Protein 2, Transcriptional Coactivator P52/P75, PAIP, P52, P75, PSIP1.
Appearance
Sterile Filtered clear solution.
Purity

Greater than 95.0% as determined by SDS-PAGE.

Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Recombinant Human PSIP1 produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 71 kDa. 
PSIP1 is expressed with a 10xHis tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
PC4 and SFRS1 Interacting Protein 1 (PSIP1), also known as LEDGF/p75, is a transcriptional coactivator that plays a crucial role in various cellular processes, including neuroepithelial stem cell differentiation, neurogenesis, lens epithelial cell gene regulation, stress responses, and lentiviral integration. It is particularly important for lens epithelial to fiber cell terminal differentiation. PSIP1 also exhibits protective effects against stress-induced apoptosis and serves as a cellular cofactor for lentiviral integration.
Description
Recombinant Human PSIP1, expressed in SF9 insect cells, is a glycosylated polypeptide chain with a calculated molecular mass of 71 kDa. It is engineered with a 10xHis tag at the N-terminus to facilitate purification, which is achieved through proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered, clear solution.
Formulation
PSIP1 is supplied in a buffer consisting of 20mM HEPES (pH 8.0), 200mM NaCl, and 20% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For long-term storage, freeze at -20°C. Repeated freezing and thawing should be avoided.
Purity
Purity is determined to be greater than 95.0% using SDS-PAGE analysis.
Immunological Functions
1. Demonstrates binding affinity to human IgG type auto-antibodies. 2. Exhibits reactivity in immunodot assays with both positive and negative control samples.
Synonyms
PC4 and SFRS1 Interacting Protein 1, Lens Epithelium-Derived Growth Factor, Transcriptional Coactivator P75/P52, Dense Fine Speckles 70 KDa Protein, CLL-Associated Antigen KW-7, DFS 70, PSIP2, DFS70, LEDGF, PC4 And SFRS1 Interacting Protein 2, Transcriptional Coactivator P52/P75, PAIP, P52, P75, PSIP1.
Source
Sf9 insect cells.

Product Science Overview

Gene and Protein Structure

The PSIP1 gene is located on chromosome 9 and encodes multiple isoforms through alternative splicing. The two main isoforms are p75 and p52, which have distinct functions but share a common N-terminal region. The p75 isoform is a transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis, while the p52 isoform acts as an adapter to coordinate pre-mRNA splicing .

Biological Functions

PSIP1/LEDGF plays a crucial role in various biological processes:

  1. Transcriptional Regulation: PSIP1 acts as a transcriptional coactivator, enhancing the transcription of specific genes by RNA polymerase II. It binds to DNA and interacts with other transcription factors to regulate gene expression .
  2. Stress Response: PSIP1 is involved in cellular stress responses, particularly in protecting cells from stress-induced apoptosis. It helps maintain cell viability under stressful conditions .
  3. Cell Differentiation: PSIP1 is essential for the differentiation of lens epithelial cells into fiber cells, a critical process in lens development. It also plays a role in neurogenesis and the differentiation of neuroepithelial stem cells .
Tissue Distribution

PSIP1 is ubiquitously expressed in various tissues, with higher expression levels observed in the brain, heart, and testis. Its expression is also noted in tumor cells, where it may contribute to tumor growth, proliferation, and angiogenesis .

Clinical Significance

PSIP1 has been implicated in several diseases and conditions:

  1. Cancer: Overexpression of PSIP1 is associated with various cancers, including lacrimal duct cancer and lymphangiosarcoma. It may promote tumor cell growth and survival .
  2. HIV Integration: PSIP1 serves as a cellular cofactor for lentiviral integration, including HIV. It facilitates the integration of viral DNA into the host genome, making it a potential target for antiviral therapies .
Recombinant PSIP1

Recombinant human PSIP1 is produced using E. coli expression systems. The recombinant protein is purified and used in various research applications, including studies on transcriptional regulation, stress response, and cell differentiation. It is available in different forms, such as liquid or lyophilized powder, and is often tagged with His-SUMO for easy purification .

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